摘要
目的探讨Caspase-6,8在骨关节炎软骨细胞中的表达及意义。方法用免疫组化SP法、原位末端标记技术(TUNEL)等方法检测Caspase-6,8在22例骨关节炎患者关节软骨细胞中的表达和软骨细胞凋亡情况,并探讨基因表达与细胞凋亡之间的关系。结果在软骨细胞中Caspase-6,8的表达实验组阳性率均高于对照组(P<0.05);实验组软骨细胞凋亡程度与Caspase-6,8的表达均呈正相关(P<0.05)。结论Caspase-6,8的表达与骨关节炎发生发展有密切关系。
Objective To investigate the expression and significance of Caspase-6,8 in articular cartilage of osteoarthfitis. Methods Immunohistochemistry and TUNEL assay were used to detect the expression of Caspase-6,8 proteins in articular cartilage and the apoptosis of the articular chondrocytes cell in 22 cases. Results The positive expression rat of Caspase-6,8 protein in osteoarthritic chondrocytes were higher than that in normal chondrocytes （P 〈 0.05）. There were a positive correlation between degrees of the expression of Caspase-6,8 and chondrocytes apoptosis in osteoarthritic articular cartilage （P 〈 0.05）. Conclusion There was a consanguineous correlation between degrees of the expression of Caspase-6,8 and chondrocytes apoptosis in osteoarthritic articular cartilage, it demonstrated that the abnormal expression of Caspase-6,8 closely related to the biological behavior of OA such as occurrence, progress, et al.
出处
《深圳中西医结合杂志》
2006年第6期340-343,F0004,共5页
Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
基金
广东省医学科研基金(A2002640)
深圳市医学科研项目基金(200104090)
关键词
骨关节炎
凋亡基因
软骨细胞
CASPASE
Osteoarthritis
Apoptosis control genes
Articular chondrocytes
Caspase
