摘要
目的研究华蟾素对小鼠脾淋巴细胞分泌白细胞介素(IL)-2水平的影响。方法体外分离纯化小鼠脾淋巴细胞,PHA-P刺激其增殖分化,并和不同浓度的华蟾素共培养,双抗体夹心ABC-ELISA法检测分泌IL-2的水平。结果0.20,0.40mg·mL-1华蟾素组小鼠脾淋巴细胞分泌IL-2水平显著高于对照组,差异有显著性(P<0.05)。结论华蟾素能促进脾淋巴细胞分泌IL-2。
Objective To study the effect of cinobufacini on the IL-2 production from mouse spleen lymphocyte. Methods After the separation and purification of spleen lymphocytes from mice, spleen lymphocytes, induced by PHA-P, was co-cultured with cinobufacini. ELISA was used to detect the level of IL-2 production. Results Cinobufacini , 0.20 - 0.40 mg·mL^-1, enhanced IL-2 production from mouse spleen lymphocytes. There was significant difference （ P 〈 0.05 ） between cinobufacini and control groups. Conclusion Cinobufacini can enhance IL-2 production from spleen lymphocytes.
出处
《医药导报》
CAS
2007年第1期14-16,共3页
Herald of Medicine
