摘要
运用HPLC法测定青海不同产地4种柴胡地上部分的黄酮,为柴胡药材的综合有效利用提供依据。采用C18色谱柱以V(甲醇)∶V(0.1%H3PO4)=1∶1为流动相,流速1 mL/min,检测波长360 nm。青海产柴胡地上部分均含丰富的黄酮,其中以秦岭柴胡黄酮质量分数最高,槲皮素质量分数达到2.27%,总黄酮质量分数大于6.50%。本方法简便,对黄酮的定量准确,改变以往柴胡只使用地下部分而弃去地上部分的局面,提高柴胡药材的有效利用率。
The contents of flavones from the aerial parts of 4 kinds different locations of Bupleurum in Qinghai by HPLC were determined. Flavones were separated on a C18 column （250 mm×4.6 mm） with methanol：0.1% H3PO4 （ 1 ： 1 ） as the mobile phase, and flow rate was 1.0 mL/min. Flavones were detected at UV 360 nm. There are abundant flavones in the aerial parts of Umbelliferae from Qinghai. The content of quercetin is 2.27 % and the contents of flavones are more than 6.50%. The method is convenient and very precise for determining the contents of flavones. The results of the study provided effective evidence for better use of umbelliferae and change the present situation that only underground parts are used and the earial parts are discarded. The abundant flavones of the aerial parts of umbelliferae can increase efficient use of this herb.
出处
《分析试验室》
CAS
CSCD
北大核心
2007年第1期46-49,共4页
Chinese Journal of Analysis Laboratory
基金
中国科学院知识创新工程(CXLY-2002-9)项目资助
关键词
HPLC
黄酮
柴胡
HPLC
Flavone
Umbelliferae
