摘要
从菌落和细胞形态、生理生化特征、细胞脂肪酸组成及同源性分析等方面，结合使用Sensititre、BioFosun、MIDI细菌鉴定系统对0℃、5℃冷藏养殖大黄鱼货架期终点细菌相进行定性和定量研究，确定优势腐败菌。0℃、5℃冷藏优势菌为腐败希瓦氏菌，比例分别为75．5％、59．6％，在营养琼脂和胰酶解大豆胨琼脂培养基上菌落圆状隆起，边缘整齐，透明，无色至粉红色，革兰氏阴性杆菌，极生单鞭毛，大小为（0．6-0．9）μm×（1．5-3．3）μm，最适生长温度25-35℃、最适pH7-9。菌株氧化酶和H2S呈阳性，能还原TMAO、液化明胶和Tween40，利用N-乙酰基-D-葡萄糖胺等有机物。菌体主要脂肪酸为16：1ω7c、iso-15：0、17：1ω8c、16：0，比例分别为21．31％、13．63％、9．82％、9．76％。菌株测试特征与细菌鉴定系统数据库比照，确定优势腐败菌为腐败希瓦氏菌。
Bacterial flora and identification of dominated spoilage organisms on cultured Pseudosciaena crocea at the end of shelf life during storage at 0 ℃ and 5 ℃ were qualitatively and quantitatively investigated, based on the colony and cellular morphology, biochemical and physiological characteristics as well as whole-cell fatty acid composition, and applied with Sensititre,BioFosun,MIDI bacteria identification systems. The dominated bacteria of Shewanella putrefaciens were 75.5% and 59.6% at the end of shelf life at 0 ℃ and 5 ℃, and its colonies on nutrition agar and trypticase soy broth agar were global apophysis, trim side, smooth clarity, achromatism to pink. The cells were gram negative rode （0.6 - 0.9）μm× （ 1.5 - 3.3）μm] and motile by means of polar flagellum, the optimum growth occurred at 25 - 35 ℃ and pH 7 - 9. Swains were positive for oxidation, H2S, TMAO, gelatin and Tween 40. SWains utilized N- acetyl- D- glucosamine, ect. The dominant fatty acids were 16： 1ω8c, iso- 15 ： 0,17： 1ω8c, 16： 0, mean contents were 21.31% ,13.63%,9.82%,9.76%, respectively. Results from tested swains were compared with database values in the bacterial identification systems, indicating the dominated spoilage organisms were Shewanella putrefaciens.
出处
《水产学报》
CAS
CSCD
北大核心
2006年第6期824-830,共7页
Journal of Fisheries of China
基金
农业部948项目(2001-478)
关键词
大黄鱼
细菌相
优势腐败菌
腐败希瓦氏菌
Pseudosciaena crocea
bacterial flora
dominant spoilage organisms
Shewanella putrefaciens
