摘要
利用套叠PCR技术对可溶性TRAIL基因片段(sTRAIL,114aa-281aa)的“KEX2”位点及“ATTTA”位点分别进行改造,并构建真核表达载体;结果表明:成功获得了3种突变基因:sTRAILK、sTRAILA以及sTRAILAK,并成功构建了4种分泌型酵母表达载体:pPICZα-A-sTRAILK,pPICZα-A-sTRAILA,pPICZα-A-sTRAILAK以及pPICZα-A-sTRAIL.
SOEing technique was used to modify the ＂KEX2＂ site and ＂ATTTA＂ site of the soluble TRAIL gene fragment （114aa-281 aa） and to construct eukaryotic expression vector. The results indicate that three kinds of modified sTRAIL genes which contain KEX2 mutation（sTRAILK） or ATTTA mutation（sTRAILA） or both of the two（sTRAILAK） and four kinds of recombinant expression vectors which are pPICZ.-A-sTRAIL, pPICZα-A-sTRAILK, pPICZα-A-sTRAILA and pPICZα-A-sTRAILAK, were successfully obtained.
出处
《生命科学研究》
CAS
CSCD
2006年第4期309-312,共4页
Life Science Research
基金
海南省教育厅科研基金项目(Hjkj200507)
