摘要
目的:研究模拟失重对大鼠血清和肺组织中黏附分子-1的影响。方法:-30°尾部悬吊(TS)大鼠模拟失重生理效应。40W istar大鼠随机分为7 d对照组(7 d CON)、21 d CON、TS 7 d和TS 21 d 4组。分别采用酶联免疫吸附试验法(ELISA)和免疫组织化学法检测血清及肺组织中黏附分子-1(ICAM-1)的动态表达。结果:TS 7 d和TS 21 d大鼠血清中ICAM-1及CON均未检测到。但TS 7 d大鼠肺组织中的细胞间黏附分子-1的活化较CON非常显著变化,有统计学意义(P<0.01),TS 21 d大鼠也有升高趋势。结论:尾部悬吊模拟失重对大鼠肺组织ICAM-1的表达产生影响,为了解失重状态肺脏的改变提供了一些依据。
Objective： To investigate the effects of simulated weightlessness on intercellular adhesion molecule-1 （ICAM-1） in serum and pulmonary tissues in rats. Methods： -30° tail-suspension（TS） rats were used as the model to simulate the physiological effects of weightlessness. 40 Wistar male rats were ：randomly arranged into 4 groups： control for 7 d （ 7 d CON）, 21 d CON, TS for7 d（TS7 d） and TS21 d. The dynamic expresses of ICAM-1 in the sera and lung tissues were respectively observed by using ELISA and the immunohisochemical technique. Results：The ICAM-1 express was not detected in sera among all groups. The activation level of ICAM-1 in the lung tissue of TS7d rats increased highly significantly, and did slightly in TS 21 d （ P 〈 0.01 ）. Conclusion： TS resulted in some lung injury of rats, which could have some effects on the pulmonary circulation and function.
出处
《军医进修学院学报》
CAS
北大核心
2006年第6期401-403,共3页
Academic Journal of Pla Postgraduate Medical School
基金
军队医药卫生十一五科研基金专项课题(06Z048)
军队医药卫生十五科研基金重点课题(04Z020)
武器装备预研基金项目(51400020104
JB1001)
关键词
失重模拟
大鼠
血清
肺
血管细胞黏附分子-1
weightlessness simulation
rat
serum
lung
vascular cell adhesion molecule-1
