摘要
目的观察乙酰半胱氨酸(NAC)对糖尿病大鼠心肌缺血再灌注(I/R)损伤的影响。方法72只大鼠分为正常假手术组(CS)、正常I/R组(CI/R)、正常I/R+NAC组(CN)、糖尿病假手术组(DS)、糖尿病I/R组(DI/R)、糖尿病I/R+NAC组(DN)。记录心电图、计算左心室发展压(LVDP)、收缩期和舒张期左心室内压的最大变化速率(±dp/dtmax),检测肌酸激酶同工酶(CK-MB)活性和丙二醛(MDA)含量。结果I/R后,CN组和DN组LVDP和±dp/dtmax高于对应的CI/R组和DI/R组,心律失常评分(AS)、CK-MB和MDA低于CI/R组和DI/R组,而DI/R组LVDP和±dp/dtmax低于CI/R组,AS、CK-MB和MDA值高于CI/R组。DN组LVDP和±dp/dtmax低于CN组,而AS、CK-MB和MDA高于CN组。结论糖尿病大鼠I/R后心功能受损程度比正常大鼠严重,补充NAC可以改善心功能,但疗效不如非糖尿病组。
Objective To evaluate the effects of N-acetylcysteine on I/R-induced myocardial injury in diabetic rats. Methods Seventy-two rats were randomly divided into six groups： non-diabetic sham operation group（CS ,12 cases）, non-diabetic I/R group （CI/R, 12 cases）, non-diabetic rats treated with NAC group （CN, 12 cases）, diabetic sham operation group（DS, 12 cases）, diabetic I/R group（DI/R ,12 cases）, diabetic rats treated with NAC group （DN, 12 cases） . Diabetes melitus was induced by intraperitoneal injection of streptozotocin（STZ） 55mg/kg. One week after STZ injection the animals in group CN and group DN were treated with NAC 100mg/kg by intragastric administration（twice per day） for 7d. The severity of arrhythmia was quantified by arrhythmia score（AS）. Left ventricular developed pressure（LVDP） and ±dp/dtmax were measured and recorded using computerized data acquisltion system throughout the experiment. Creatine klnase isoenzyme-MB（CK-MB） and myocardial malondialdehyde （MDA） were measured after the experiment finished. Results After I/R, the values of AS, CK-MB and MDA were significantly higher in CN group than those in Cs group but lower than those in CI/R group, while LVDP and ±dp/dtmax were significantly lower than those in Cs but higher than those in CI/R group. LVDP and ±dp/dtmax in DN group were significantly higher than those in DI/R group but lower than those in CN group, while the AS,CK-MB and MDA were significantly lower in DN group than those in DI/R group but higher than those in CN group. Conclusion Diabetic rat hearts are more susceptible to I/R-induced arrhythmia and cardiac dysfunction. NAC can improve the recovery of cardiac function and reduce the severity of I/R- induced arrhythmia in both non-diabetic rats and diabetic rats, but the therapeutic effects are less effective in diabetic rats than those in non-diabetic rats.
出处
《重庆医学》
CAS
CSCD
2007年第1期50-52,共3页
Chongqing medicine
基金
本课题受教育部留学回国人员科研启动基金资助[教外司留(2003)14]
