摘要
目的探讨C-ERBB2对小鼠卵母细胞成熟的影响及对表皮生长因子在卵母细胞成熟调控中的作用。方法采用卵母细胞体外培养法,研究反义C-ERBB2寡脱氧核苷酸(C-ERBB2ASODN)对卵母细胞体外自发成熟及对表皮生长因子(EGF)促卵母细胞成熟的影响;利用RT-PCR检测卵母细胞中C-ERBB2mRNA的表达及在不同处理因素作用下卵母细胞成熟过程中C-ERBB2mRNA表达的变化。结果C-ERBB2ASODN能呈剂量依赖方式抑制卵母细胞的生发泡破裂(GVBD)率和第一极体(PBⅠ)排出率,并且能抑制EGF对卵母细胞的促成熟作用。卵母细胞中存在C-ERBB2mRNA,EGF可以增强其表达,C-ERBB2ASODN可减弱EGF促表达的作用。结论C-ERBB2与卵母细胞的成熟密切相关。EGF可直接促进裸卵的成熟分裂,其机制可能与上调卵母细胞C-ERBB2的表达有关。
Objective Potential roles of the C-ERBB2 protooncogene in mouse oocyte maturation and in conducting Epidermal Growth Factor （EGF） promoteing mouse oocyte maturation in vitro were investigated. Methods This research used mouse oocyte culture model and RT-PCR method to study the effects of both C-ERBB2 and EGF on mouse oocyte maturation in vitro, and the potential role of the C-ERBB2 protooncogene on EGF effect. Results C-ERBB2 ASODN inhibited germinal vesical breakdown（GVBD） and the first polar（ PB Ⅰ） extrusion of denuded oocytes（DOs） in a dose-dependent and time-dependent way, and delayed their maturation significantly. C-ERBB2 ASODN also inhibited the effect of EGF on oocyte maturation in a time-dependent way. In GVBD oocytes, RT-PCR showed that C-ERBB2 mRNA was expressed in oocytes and the level of C-ERBB2 mRNA was the highest in EGF treated group, midst in EGF ＋ c-erbB2 ASODN treated group, and lowest in C-ERBB2 ASODN treated group. Conclusion C-ERBB2 exists in the oocyte and has promoting effect on oocyte maturation in vitro. EGF plays a role in inducing denuded oocyte maturation through up-regulating C-ERBB2 expression of oocyte. Interfering C-ERBB2 expression can reduce the effect of EGF on oocyte maturation.
出处
《基础医学与临床》
CSCD
北大核心
2006年第10期1056-1062,共7页
Basic and Clinical Medicine
基金
国家自然科学基金(30260035)
江西省卫生厅课题(20042014)
