摘要
本文用无水乙醇提取鸡蛋中的胆固醇，同时沉淀蛋白质，向提取液中加入高铁—磷酸—硫酸（硫磷铁）显色剂，胆固醇与浓硫酸及三价铁作用，生成较稳定的紫色化合物。测定波长为５５０ｎｍ，胆固醇含量在０～８０μｇ／４ｍｌ范围内服从比尔定律，相对标准偏差为０．８２％，回收率在９８％～１０１％之间。本方法简便易行，测定鸡蛋中胆固醇的含量能获得满意的结果。
In this experiment,the cholesterol in eggs is extracted by alcohol with precipitating albumin,and then ferric chlorine-phosphoril and sulphuric acids (S-P-Fe) reagents are added to the extracted alcohol.The reaction of cholesterol with H2SO4-Fe3+ produces a more stable compound.It is shown that the reaction coincides Beer' s law under the wavelength of 550nm and the concentration of cholesterol from 0 to 80ug/4ml.The relative standard deviation of the results is 0.82%,and the recovery ratio of cholesterol is 98%～101%.So it is indicated that this method is simple and available for determining the cholesterol in eggs with satisfactory results.
关键词
鸡蛋
胆固醇
硫磷铁试剂
测定
Egg cholesterol
Ferric Chlorine-phosphoric and Sulphuric acids
