摘要
目的:建立山楂属植物叶的HPLC指纹图谱,对该属植物中所含的化学成分进行综合评价,并为该属植物叶鉴定提供依据。方法:对10个产地山楂叶进行分析,以绿原酸为内参照物,检测它们所含牡荆素,金丝桃苷,牡荆素鼠李糖苷,芦丁,鼠李糖芦丁,槲皮素,葡萄糖牡荆素的含量。结果:不同种山楂叶均显示了它们各自的指纹特征。结论:HPLC指纹谱可以用来鉴别山楂属的不同种植物叶,该属植物种间的化学成分差别较大,而且主要特征性成分的含量也存在明显差异。
AIM： To establish HPLC fingerprint chromatogram of leaves of Hawthorn and to evaluate the con- stituents and to provide the basis for identification. METHODS： Leaves of Hawthorn come from 10 production places were analysed to gain its contents, such as Vitexin, Hyperoside, 2＂-O-Rhamnosylvitexin, Rutin, 4＂-O-Rham- nosylrutin, Chlorogenic acid, Quercetin 3-O-13-glucoside ,4＂-O-Glucosylvitexiu, and internal reference selected Chlorogenic acid. RESULTS： The different leaves of Hawthorn showed different HPLC chromatogram. CONCLUSION： HPLC chromatogram can be used to distinguish the leaves of different species of Hawthorn and the content of the main characteristic components are various as production places vary.
出处
《中成药》
CAS
CSCD
北大核心
2007年第1期7-11,共5页
Chinese Traditional Patent Medicine
基金
国家自然科学基金资助项目(No.30472161)
关键词
山楂叶
HPLC
指纹图谱
Hawthorn leaves
HPLC
fingerprint chromatogram
