摘要
以法国孟士德薰衣草(Lavandula angustifoliacv.Munstead)的下胚轴和子叶为研究材料,通过高频再生系统的建立和抗生素敏感性实验,建立了薰衣草基因转化的直接分化受体系统。结果表明:下胚轴的不定芽分化能力比子叶更强,在IAA 0.1 mg.L-1+6-BA 1.0 mg.L-1条件下,10 d苗龄的下胚轴的出愈率和不定芽分化率分别为96.7%和36.7%;所得组培苗在1/2 MS基本培养基上生根率可达95%以上;确定了下胚轴适宜的抗生素筛选浓度,潮霉素的筛选浓度为10 mg.L-1,头孢霉素浓度为200 mg.L-1时即可有效抑制农杆菌的生长,同时又不会对薰衣草不定芽分化产生明显的伤害作用。
The essential oil of lavender is extensively used in pharmacy, food processing, cosmetic production, etc. Xinjiang is the main lavender-growing region in China, the lavender-growing area is 1 400 hm^2, and the annual yield of essential oil of lavender is more than 100 t and occupies over 95 % of the total in China. However, the idioplasm of lavender in Xinjiang is degenerated because Xinjiang is located in the hinterland of Eurasia, the climate is arid, and the diurnal-nocturnal and seasonal temperature difference is high. Therefore, it is urgent to breed new varieties of lavender, which are adaptable to the climatic conditions in Xinjiang and can be grown in large areas. With hypocotyls and cotyledons of Lavandula angustifolia cv. Munstead as the study subject, in this study a high-frequency regeneration system is constructed, the experiments about antibiotic sensitivity are conducted, and the acceptor system of direct differentiation for the gene transformation of lavender is developed. The results reveal that the shoot differentiation rate of hypocotyls is higher than that of cotyledons. Under the conditions of IAA 0.1 mg·L^-1 ＋ 6-BA 1.0mg·L^-1, the calli formation rate and adventitious shoot differentiation rate of hypocotyls from 10-day-aged plantlets are 96.7% and 36.7% respectively. The resulted adventitious shoots are easily rooted on 1/2 MS medium, and the rooting rate is higher than 95 %. The concentration of feasible antibiotics for lavender is determined, the concentration of hygromycin is 10 mg· L^-1, the concentration of cefotaxime 200 mg·L^-1 is harmless to the shoot differentiation of lavender, but is enough to inhibit the growth of agrobacterium.
出处
《干旱区研究》
CSCD
北大核心
2007年第1期23-27,共5页
Arid Zone Research
基金
中国科学院西部行动高新技术项目(KGCX2-SW-506)
中国科学院百人计划项目(2002)
