摘要
鹅掌楸是优良绿化与优质用材的野生珍稀植物之一,采野生多年生植物的冬芽为外殖体,以春芽、叶片、叶柄、花蕾和果实作对照,用MS为基本培养基,以1/2MS作对比,研究鹅掌楸的组织培养快繁技术。结果表明,初代培养最适为:MS+2.5 mg/L6-BA+1.0 mg/L IBA+0.2 mg/L KT,增殖与继代培养最适为:MS+1.0 mg/L6-BA+0.5 mg/L IBA;将组织培养苗扦插在有机腐殖质土和珍珠岩(3:1)基质中,瓶外生根率达83.3%以上。
Liriodendron Chinense is a rare wild plant that is used for fine afforestation and high - quality wood. Winter buds of its perennial plant are taken for the explant. Meanwhile, spring buds, blades, petioles ,flower buds and fruits are companY, The MS is regarded as basic medium and 1/2MS is compared. Vitro rapid micropropagation technology of Liriodendron ehinense is researehed. The results showed that best medium for first culture is MS ＋ 6 - BA2.5mg/L ＋ IBA1.0mg/L ＋ KT0. 2 mg/L. The best medium for proliferation and inherit culture are MS ＋ 6 - BA1. 0mg/L ＋ IBA2.0mg/L, The striking root rate outside the bottle of young plant that is cuttaged in admixture （ soil of organic humus ： pearlite = 3 ： 1 ） is more 83.3%.
出处
《黔南民族师范学院学报》
2006年第6期58-60,77,共4页
Journal of Qiannan Normal University for Nationalities
关键词
鹅掌楸
组织培养
离体快繁
Liridendren chinense, Tissue culture ,vitro rapid Microprepagation
