摘要
为探讨ｂｃｌ－２蛋白对足叶乙甙触发Ｔ淋巴细胞白血病细胞株ＣＥＭ程序性死亡的调控作用，采用脂质体Ｌｉｐｏｆｅｃｔｉｎ法，将ｂｃｌ－２基因逆转录病毒载体转入人Ｔ淋巴细胞白血病细胞株ＣＥＭ中，并使其稳定、高效地表达。结果，经足叶乙甙处理后，高表达ｂｃｌ－２蛋白的ＣＥＭ细胞产生梯状ＤＮＡ的量低于对照（Ｐ＜０．０５）。这表明高水平ｂｃｌ－２蛋白对白血病细胞程序性死亡过程可能具有较强的抑制效应。
By using lipofectin transduction,human T lymphocytic leukemia cell line CEM was successfully transfected with retroviral vector containing bcl-2 gene,resulting in a highly efficient and stable expression of the target gene.After etoposide exposure,the ladder DNA produced by the bcl-2 transfected CEM cells reduced by approximately 20% as compared with control(P<0.05).The data showed that high level of bcl-2 protein might have stronger inhibitive effect on programmed cell death of leukemic cells.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1996年第10期538-540,共3页
Chinese Journal of Hematology
基金
国家自然科学基金
关键词
BCL-2蛋白
白血病
细胞株
T淋巴细胞
Bcl-2 protein Leukemia cell line(CEM)Etoposide Programmed cell death
