摘要
以薄荷(Mentha haplocalyx Briq)幼嫩叶片为外植体,采用正交设计L9(33)考察6-BA,NAA,2,4-D3种植物生长调节物质对叶片愈伤组织诱导的影响,实验结果表明薄荷叶片愈伤组织诱导最佳培养基为MS+6-BAl.0mg/L+NAAl.0mg/L+2,4-D0.5mg/L+蔗糖30g/L.在此培养基上研究不同培养条件对愈伤组织增殖效应的影响,结果表明薄荷愈伤组织继代增殖适合且经济的培养条件为蔗糖20g/L,液体浅层静置培养.
Yong leaves of Mentha haplocalyx Bfiq were used as explants. Effect of three kinds of plant regulators （6-BA,NAA, 2,4-D） were investigated using orthogonal test design method.The results showed that the optimal medium was MS＋6-BA1.0 mg/L＋NAA1.0 mg/L42,4-D0.5 mg/L＋suemse30g / L.The study on different euhure condition of callus pfiliferation shown that 五tandeeonomiealeonditionwere20/L suemse,liquidshallow quieseenteuhure.
出处
《黔东南民族师范高等专科学校学报》
2006年第6期42-43,共2页
Journal of Southeast Guizhou National Teachers College
关键词
薄荷
愈伤组织
诱导
增殖
Mentha haplocdyx Bfiq
callus
inductin
proliferation
