摘要
目的：探讨Ｐ５３基因突变表达产物与卵巢肿瘤临床生物学行为之间的关系．方法：采用单克隆抗体免疫组化染色方法对５０例卵巢恶性肿瘤，１０例良性肿瘤的石蜡切片标本进行Ｐ５３基因突变蛋白表达检测．结果：卵巢恶性肿瘤Ｐ５３阳性表达率２４％（１２／５０），卵巢良性肿瘤均为阴性卵巢上皮癌阳性结果为２９％（８／２８），生殖细胞瘤１０％（１／１０），间质瘤２５％（２／８），转移瘤２５％（１／４）；不同临床分期阳性表达：Ⅰ期４例，Ⅱ期３例，Ⅲ期２例，Ⅳ期３例；Ｐ５３免疫组化染色阳性家系有癌症史３例（３／１２），阴性者１例（１／３８）．结论：结果表明①各种组织类型的卵巢恶性肿瘤均可出现Ｐ５３阳性表达，似与组织分型无关（Ｐ＞０．５）；②Ｐ５３阳性表达与临床分期无关（Ｐ＞０．２５）；③提示Ｐ５３阳性表达可能与遗传因素有关（Ｐ＝０．０３８）；④Ｐ５３阳性表达与卵巢恶性肿瘤的淋巴结转移未发现明显的相关性．
Objective: To find out the relationship between P53 gene mutation protein product and clinical biological behavior of ovarian malignance. Methods: We used monoclonal antibody immunohistochemical staining method and examined the paraffin embedded sections of 50 cases of ovarian malignance and 10 cases of benign ovarian tumors for P53 gene mutation protein product. Results: Positive expression rate of P53 for ovarian malignance was 24% (12/50) and the expression rate for benign tumors was negative. Positive results in different cell type tumors were: epithelial cell type 29% (8/28), genetic cell type 10% (1/10), interstitial cell type 25% (2/8) and metastatic tumors 25% (1/4). Positive results in different clinical stages were: 4 cases in stage Ⅰ, 3 cases in stage Ⅱ, 2 cases in stage Ⅲ and 3 cases in stage Ⅳ. Family history revealed that 3 of the 12 positive patients had victims of tumor growth, while only one of the 38 negative P53 patients and family history of malignant growth. Conclusions are drawn as follows: (1) Positive results can be found in various pathological types , which seems to show no relation to the types ( P >0.5). (2) Positive results of P53 has nothing to do with clinical stages ( P >0.25). (3) Positive results of P53 may be correlative with inheritance ( P =0.038). (4) No obvious correlation was found between positive results of P53 and lymphatic metastasis of ovarian malignance.
出处
《第四军医大学学报》
1996年第5期340-342,共3页
Journal of the Fourth Military Medical University
