参附注射液预处理对大鼠心肌缺血再灌注损伤后HSP70基因表达的影响被引量：10

Effects of Shenfu Injection Expression of HSP70 mRNA and its Protein in Rats with Myocardial Ischemia Reperfusion Injury

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摘要目的:探讨参附注射液预处理对心肌缺血再灌注损伤的保护作用及机制。方法:30只Wistar大鼠随机分为3组:假手术组、缺血再灌注损伤(IR)组、参附注射液预处理组(SFI)。建立大鼠心肌缺血再灌注损伤模型,应用原位杂交技术和免疫组化S-P法检测HSP70mRNA及其蛋白的表达。结果:与缺血再灌注损伤(IR)组比较,参附注射液预处理组(SFI)HSP70mRNA(P<0.01)及其蛋白(P<0.05)的表达显著增加。结论:HSP70是心肌中重要的内源性保护物质,参附注射液可诱导HSP70mRNA及其蛋白的表达而发挥心肌保护作用。 Objective： To investigate the protective effects and its mechanism of shenfu injection on myocardial ischemia reperfusion injury rats. Method： 30 Wistar rats were divided into three groups randomly： sham operation group, ischernia reperfusion injury group （IR）, and shenfu injection preconditioning group （SFI）. The model of myocardial ischemia reperfusion injury was produced. In situ hybridization assay and immunohistochcmical S- P method were used to detect the expression of HSP70 mRNA and its protein in 30 cases ischemia reperfusion injury myocardial tissues. Result： The expressions of HSP70 mRNA （ P 〈 0.01） and its protein （ P 〈 0.05） were significantly higher in shenfu injection preconditioning group （SFI） than that in ischemia reperfusion injury group （IR）. Conclusion： HSP70 are important substabces of endogenous peotection, and shenfu injection can enehance the expressions of HSP70 gene and protect myocardial ischemia reperfusion injury.

作者王峰尚立芝曹珊崔明霞

机构地区河南中医学院河南省胸科医院

出处《河南中医学院学报》 2007年第1期36-38,共3页 Journal of Henan University of Chinese Medicine

关键词参附注射液缺血再灌注损伤 HSP70 原位杂交技术免疫组化 Shenfu injection Iisehemia reperfusion injury HSP70 In situ hybridization immunohistochemistry

分类号 R285.5 [医药卫生—中药学]

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