摘要
肝炎 B 病毒(HBV ) 感染是在世界上的一个严重健康问题。然而，仍然为 HBV 感染没有令人满意的治疗学的策略。到为有更高的功效和更少的副作用的新 anti-HBV 代理人的搜索，繁体中文药感冒 palmatum L 的禁止的活动。对 HBV 复制的乙醇摘录(RPE ) 在这研究被调查。量的即时聚合酶链反应(PCR ) 被采用在稳定的生产 HBV 房间线 HepAD38 对 HBV-DNA 复制分析 RPE 的禁止的活动；HBV 表面抗原(HBsAg ) 和 e 抗原(HBeAg ) 的表示层次被酶也决定在 RPE 处理以后的连接 immunosorbent 试金(ELISA ) 。RPE 能 dose-dependently 禁止 HBV-DNA 和 HBsAg 的生产。50% 抑制(IC50 ) 的集中在 209.63 点被计算， 252.53 渭 g /mL， respectivel y。然而，它对 HBeAg 表示的禁止的活动甚至在高集中是细微的。RPE 在 HepAD38 房间上有弱细胞毒素的效果(CC50 = 1 640 渭 g /mL ) 并且选择索引(SI ) 在 7.82 点被计算。与二 anthraquinone 衍生物 emodin 和 rhein 相比， RPE 显示出 anti-HBV 和更弱的 cytotoxicity 的更高的能力。那么感冒 palmatum L。可能拥有能有效地禁止 HBV-DNA 复制和 HBsAg 表示的另外的功能的代理人。他们改进功效并且减少的结构的活跃代理人，鉴定和修正的进一步的纯化 cytotoxicity 被要求。关键词肝炎 B 病毒(HBV )- 抗病毒 - 感冒 palmatum L.ethanol 摘录(RPE )- HepAD38 房间 CLC 数字 R373 同等地相应的作者。
Hepatitis B virus（HBV） infection is a severe health problem in the world. However, there is still not a satisfactory therapeutic strategy for the HBV infection. To search for new anti-HBV agents with higher efficacy and less side-effects, the inhibitory activities of traditional Chinese medicine Rheum palmatum L. ethanol extract（RPE） against HBV replication were investigated in this study. Quantitative real-time polymerase chain reaction（PCR） was employed to analyze the inhibitory activity of RPE against HBV-DNA replication in a stable HBV-producing cell line HepAD38; the expression levels of HBV surface antigen（HBsAg） and e antigen（HBeAg） were also determined by enzyme linked immunosorbent assay（ELISA） after RPE treatment. RPE could dose-dependently inhibit the production of HBV-DNA and HBsAg. The concentration of 50% inhibition（IC50） was calculated at 209.63,252.53 μg/mL, respectively. However, its inhibitory activity against HBeAg expression was slight even at high concentrations. RPE had a weak cytotoxic effect on HepAD38 cells（CC50 = 1 640 μg/mL） and the selectivity index（SI） was calculated at 7.82. Compared with two anthraquinone derivatives emodin and rhein, RPE showed higher ability of anti-HBV and weaker cytotoxicity. So Rheum palmatum L. might possess other functional agents which could effectively inhibit HBV-DNA replication and HBsAg expression. Further purification of the active agents, identification and modification of their structures to improve the efficacy and decrease the cytotoxicity are required.
出处
《中国病毒学》
CSCD
2007年第1期14-20,共7页
Virologica Sinica
