摘要
目的探讨天麻在大鼠肾缺血再灌注损伤中的保护作用及其机制。方法建立大鼠急性肾缺血再灌注损伤模型,测定天麻对肾缺血再灌注后血清BUN浓度、肾组织SOD活力、MDA及NO含量的变化及肾脏的病理组织学改变。结果与对照组相比,使用天麻后,在灌注24h后血清BUN浓度明显下降(P值<0.01),再灌注1、6、12和24h后肾组织SOD活力明显升高(P值分别<0.01,0.05,0.01),MDA含量明显降低(P值分别<0.05,0.01,0.05);未应用天麻保护的对照组肾组织出现明显的损伤性改变。结论天麻在大鼠肾缺血再灌注损伤模型中有保护作用,其机制可能与天麻的抗自由基损伤和减轻脂质过氧化的作用有关。
Objective To study the anti - oxidant effects of Rhizoma Gastrodiae on rat mode＇s with renal ischemia repetfusion injuries. Methods Rhizoma Gastrodiae was given intrapefitoneally to rat for every 6 hours beginning from 2 days before surgery until 1,6,12 and ,24 hours after reperfusion. At 1, 6, 12 and 24 hours after reperfusion, SOD and MDA in renal tissues were deter. mined, and at 6 and 24 hours after reperfusion renal NO level and serum concentration of BUN were analyzed. Results 6, 12 and 24 hours after reperfusion, the kidney SOD activities were significantly increased （ P 〈 0.01, P 〈 0.05 and P 〈 0.01, respectively） ,while the concentration of renal MDA significantly decreased （ P 〈 0.05, P 〈 0.01 and P 〈 0.05, respectively）. Serum BUN level was significantly declined at 24 hours after reperfusion （ P 〈 0.01 ）. Conclusion Rhizoma Gastrodiae may have preventive role in renal ischemia repeffusion injury, possibly through anti - oxidative actions against free radicals and lipid peroxides.
出处
《中国热带医学》
CAS
2007年第2期187-189,共3页
China Tropical Medicine
关键词
天麻
大鼠
肾
缺血再灌注
保护作用
Rhizoma Gastrodiae
Rat
Kidney
Renal ischemia repcrfusion
Antioxidation effect
