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HIV-1感染对T和B细胞核内UNG2 mRNA表达的影响 被引量：3

Up-regulation of human nuclear form of uracil-DNA glycosylase mRNA in HIV-1 infected human peripheral T cells and C8166 cells
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摘要 目的:探讨HIV-1感染是否影响细胞中UNG2的表达。方法:采用四步法SYBR greenⅠ实时定量RT-PCR,对HIV-1感染者的T和B淋巴细胞,以及HIV-1感染的C8166细胞核内UNG2 mRNA的表达进行测定。结果:UNG2 mRNA的表达在HIV-1感染者的T细胞和HIV-1感染的C8166细胞中被明显上调,分别是对照的8.76倍和8.14倍,而在HIV-1感染者的B细胞中却没有被上调。结论:HIV-1感染导致的UNG2表达上调,可能通过减少TCR的多样性削弱Th的功能,另一方面可能有利于病毒对UNG2的包装。 Objective： To investigate whether HIV-1 infection may activate the expression of nuclear UNG2 mRNA of T and B cells, which may inhibit antibody gene hypermutation and decrease antibody di- versity. Methods： The 4-step program in SYBR green I real-time RT-PCR was used to quantify the expression of UNG2 mRNA in T and B cells of HIV-l-infeeted individuals, and in HIV-1-infeeted C8166 cells. Results： The expression of UNG2 mRNA in T cells from HIV-1 infected patients and in C8166 cells infected with HIV-1 was found to be obviously up-regulated. Their relative expression indices were 8.76 and 8.14 in comparison with those of uninfected controls, respectively. Conclusion： The increased UNG2 in HIV-1 infected cells may lead to weakening Th cell function by decreasing TCR diversity, and may benefit incorporation of this enzyme into HIV-1 particles.
作者 张驰宇 许燕萍 贲昆龙
机构地区 江苏大学医学技术学院 中国科学院昆明动物研究所
出处 《江苏大学学报（医学版）》 CAS 2007年第1期5-8,共4页 Journal of Jiangsu University：Medicine Edition
基金 江苏省高校自然科学指导性计划资助项目(04KJD180044) 江苏大学高级技术人才科研启动基金资助项目(2281270002)
关键词 尿嘧啶DNA糖苷酶 HIV-1 T细胞受体 免疫逃逸 超突变 uracil-DNA glycosylase HIV-1 T cell receptor immune escape somatic hypermutation
分类号 Q5 [生物学—生物化学] R512.91 [医药卫生—内科学]
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参考文献20

  • 1Kvaloy K, Nilsen H, Steinsbekk KS, et al. Sequence variation in the human uracil-DNA glycosylase (UNG)gene[J].Mutat Res, 2001,461(4) :325 -338.
  • 2Nilsen H, Otterlei M, Haug T, et al. Nuclear and mitochondrial uracil-DNA glycosylases are generated by alternative splicing and transcription from different positions in the UNG gene[J]. Nucleic Acids Res, 1997,25(4) :750 -755.
  • 3Muramatsu M,Sankaranand VS, Anant S, et al. Specific expression of activation-induced cytidine deaminase(AID), a novel member of the RNA-editing deaminase family in germinal center B cells [ J ]. J Biol Chem,1999, 274 (26) : 18470 - 18476.
  • 4Muramatsu M, Kinoshita K, Fagarasan S, et al. Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme[J]. Cell, 2000, 102(5) :553 -563.
  • 5Storb U,Stavnezer J. Immunoglobulin genes: generating diversity with AID and UNG[J].Curr Biol, 2002, 12(21) : R725 -727.
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二级参考文献36

  • 1徐顺高,黄新祥,周丽萍.荧光实时定量RT-PCR观察伤寒杆菌鞭毛z66和d/j抗原基因表达方法的建立[J].江苏大学学报（医学版）,2005,15(1):21-24. 被引量：3
  • 2Lindahl T. New class of enzymes acting on damaged DNA[J]. Nature, 1976, 259(5538): 64-66.
  • 3Lindahl T, Ljungquist S, Siegert W, et al. DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli[J]. J Biol Chem, 1977, 252(10):3286 - 3294.
  • 4Bhagwat AS. DNA-cytosine deaminases: from antibody maturation to antiviral defense [ J ]. DNA Repair (Amst), 2004, 3(1): 85-89.
  • 5Chen R, Wang H, Mansky LM. Roles of uracil-DNA glycosylase and dUTPase in virus replication[ J ]. J Gen Virol, 2002, 83( 10): 2339-2345.
  • 6Nilsen H, Otterlei M, Haug T, et al. Nuclear and mitochondrial uracil-DNA glycosylases are generated by alternative splicing and transcription from different positions in the UNG gene[ J]. Nucleic Acids Res, 1997,25(4): 750 -755.
  • 7Kvaloy K, Nilsen H, Steinsbekk KS, et al. Sequence variation in the human uracil-DNA glycosylase (UNG)gene[J]. Mutat Res, 2001,461(4): 325-338.
  • 8Muramatsu M, Kinoshita K, Fagarasan S, et al. Class switch recombination and hypermutation require activation-induced cytidine deaminase ( AID ), a potential RNA editing enzyme[J]. Cell, 2000, 102(5): 553 -563.
  • 9Muramatsu M, Sankaranand VS, Anant S, et al. Specific expression of activation-induced cytidine deaminase (AID) , a novel member of the RNA-editing deaminase family in germinal center B cells [ J ]. J Biol Chem,1999, 274(26): 18470 - 18476.
  • 10Storb U, Stavnezer J. Immunoglobulin genes: generating diversity with AID and UNG[J]. Curr Biol, 2002, 12(21): R725 -R727.

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江苏大学学报（医学版）
2007年 第1期

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