摘要
根据编码猪伪狂犬病病毒gH基因保守序列,设计合成一对引物,通过改进病毒核酸提取方法和优化PCR反应条件,成功的从猪伪狂犬病毒感染的细胞中扩增出预期的355bp片段,而猪繁殖与呼吸综合征病毒、猪细小病毒、猪瘟病毒、猪乙型脑炎病毒和正常细胞均未扩增出相应的片段,经NaeⅠ酶切鉴定,证实了该扩增片段的特异性;敏感性实验表明,该体系可检测到0.48pg的猪伪狂犬病毒DNA。本方法的建立使猪伪狂犬病病毒的检测更为快速、简便、经济、实用。
A PCR based detection method was developed using a pair of primers designed from a conserved region of the pseudorabies virus（PRV） gH gene. This method uses an improved DNA extraction approach to isolate DNA from PRV infected PK-15 cells and optimized PCR conditions to detect the presence of PRV sequence. A 355 bp fragment was amplified from PRV infected PK-15 cells, but not from PRRSV, PPV, HCV, JEV and PK-15 cells. This amplified fragment is specific for PRV DNA based on Nae. Ⅰ digestion pattern. The results showed that this improved PCR approach could be a fast, simple and specific detection method for PRV.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2007年第2期142-146,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
河南省杰出人才创新基金(编号0621002100)
