摘要
目的:研究硒螺旋藻(Se enriched Spirulina platensis,Se-SP)拮抗大鼠肝纤维化效应,以及对肝细胞增殖和谷胱甘肽过氧化物酶(GSH-Px)和硫氧还蛋白还原酶(TR)活性的上调作用。方法:在SD大鼠用硫代乙酰胺诱导肝纤维化模型,同期补充Se-SP,取肝进行组织病理分析,检测肝组织脂质过氧化产物丙二醛(MDA)含量及GSH-Px和TR活性,免疫组化方法测定肝细胞增生细胞核抗原(PCNA),放射免疫法测定肝细胞3H-TDR掺入水平。结果:Se-SP可明显减轻鼠肝组织纤维化程度,比亚硒酸钠更明显地促进肝细胞硒酶活性和细胞增殖,同时可抑制肝组织胶原的生成和血清透明质酸(HA)含量。结论:Se-SP对肝纤维化具有较强的抗氧化和促肝细胞再生及功能修复作用,其机制主要是Se-SP作为高生物利用度的补硒剂,通过上调硒酶活性,发挥直接或间接的生物效用。
Objective： To explore the antagonism of selenium enriched Spirulina platensis （Se-SP） to hepatocirrhosis by enhancement of cell proliferation and selenoenzyme activity. Method： The male SD rat hepatocirrhosis model was induced by thioacetamide （TAA）, and Se-SP was supplemented. Hepatic histological analysis was performed and relative content of collagen （RCC %） was estimated using IBAS 2000 system after Masson＇s staining. Glutathione peroxidase （GSH-Px）, thioredoxin reductases （TR） and malondialdehyde （MDA） in hepatocyte as well as hyaluronic acid （HA） in serum were determined. Expression index of proliferating cell nuclear antigen （PCNA） in hepatocytes was detected by immu- nohistochemistry, and the level of synthesis of DNA in regenerative hepatocytes was analyzed by radioimmunity for incorporation of ^3H-TDR. Results： Hepatocirrhosis was induced by TAA at 9w of the expeiment, and the obvious antagonism of Se-SP to hepatocirrhosis was obsewed after Se-SP supplementation. 12.5% rats of Se-Sp group were completely recovered at 15w of the experiment （6w after withdrawal of TAA）. GSH-Px and TR activity in hepatocytes as well as PCNA and ^3H-TDR incorporation rate in hepatocytes were obviously enhanced （P 〈 0.05） with Se-SP supplemention. MDA and RCC% in hepatocytes as well as HA in serum were remarkably decreased （P 〈 0.05） with Se-SE although there were various effects at 9w and 15w. Conclusion： As an effective Se additive with high bioavailability, Se-SP performed well in antagonism to hepatocirrhosis by antioxidation, enhancing hepatocyte proliferation and reversal of cell function. The mechanism was associated with enhancement of selenoenzymes activity.
出处
《营养学报》
CAS
CSCD
北大核心
2007年第1期39-42,共4页
Acta Nutrimenta Sinica
基金
广东省自然科学基金重点项目(No.05103295)
关键词
硒
螺旋藻
肝纤维化
硒酶
细胞增殖
Spirulina platensis
selenium
hepatocirrhosis
selenoenzyme
proliferation
