摘要
目的利用体外细胞培养技术,对聚乳酸-甲壳素接骨板的细胞毒性进行研究,为临床应用作前期准备。方法分别采用间接接触法(MTT法)和直接接触法评价聚乳酸-甲壳素接骨板的细胞毒性。MTT法:采用体外培养的小鼠成纤维细胞(L929),在培养液中分别添加不同浓度的聚乳酸-甲壳素接骨板浸提液(0、50%和100%),于接种后第2、4、7天通过MTT法显色,在酶标仪450nm波长处测定吸光度值,计算相对增殖率,对聚乳酸-甲壳素接骨板的细胞毒性进行评价;直接接触法:将体外培养的L929细胞分为两组,空白对照组为常规培养的L929细胞,实验组为L929细胞与聚乳酸-甲壳素接骨板共培养,逐日用倒置显微镜和扫描电镜观察细胞形态学发生的变化,评价聚乳酸-甲壳素接骨板的细胞毒性。结果随道时间推移,不同浓度的聚乳酸-甲壳素接骨板浸提液对L929细胞增殖均有促进作用(P<0.01),在第2、4、7天各浓度之间无明显差异性(P>0.05),提示L929细胞增殖与聚乳酸-甲壳素接骨板浸提液的浓度无明显相关;L929细胞在聚乳酸-甲壳素接骨板表面粘附生长,随时间推移,粘附细胞数量增加,细胞数量及形态与空白对照组比较无明显差异,评分聚乳酸-甲壳素接骨板的细胞毒性为0级。结论聚乳酸-甲壳素接骨板无明显细胞毒性,具有良好细胞相容性,是一种具有发展前景的可吸收骨折内固定材料。
Objective To evaluate the cytotoxicity of polylactic acid-chitin plates in vitro. Methods The cytotoxicity of polylactic acid-chitin plates was evaluated with MTT assay and direct contact assay using the cell line L929 in vitro. L929 ceils were cultured with different concentrations of the extracts ofpolylactic acid-chitin plates （0, 50% and 100%, V/V） in vitro, and MTT assay was performed to evaluate the cell proliferation at 2, 4 and 7 days. The morphologic changes of the ceils were observed by inverted microscope and scanning electron microscope. Results MTT assay revealed that the extracts of polylactic acid-chitin plates enhanced the cell proliferation （P〈0.01）, but not in a dose-dependent manner （P〉0.05）. In the direct contact assay, no side effects were found in regard to the cell growth, proliferation and attachment to the polylactic acid-chitin plates as compared with the negative control. The cytotoxicity grade of polylactic acid-chitin plates was 0 or 1, suggesting that the material was almost free of cytotoxicity. Conclusion The polylactic acid-chitin plate possesses ideal cellular compatibility, and therefore has great potential for clinical use as an internal bone fixation material.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第1期65-68,共4页
Journal of Southern Medical University
基金
广东省重点攻关项目(99M04503G)~~
