摘要
目的探讨聚维酮(PVP)对人膀胱癌T24细胞的抑制作用。方法采用MTT法检测PVP对T24细胞生长的抑制作用,流式细胞仪检测PVP对T24细胞周期的影响和黏附作用。结果2.5%PVP作用24和72 h,对人膀胱癌T24细胞生长抑制率分别为(15.11±2.36)%和(49.57±7.07)%;浓度为7.5%时24和72 h抑制率分别(35.42±5.55)%和(79.66±19.92)%。5.0%PVP作用24和72 h时,细胞G0/G1期所占比例分别为(74.17±0.91)%和(46.69±3.76)%;G2/M期细胞所占比例分别为(14.63±0.47)%和(41.88±1.50)%。PVP能提高抗鼠IgG1对T24细胞的黏附作用。结论PVP能通过诱导人膀胱癌T24细胞周期阻滞于G2/M期和作为化疗药的黏附性载体而抑制癌细胞的生长增殖。
Objective To probe into the inhibitory effect of polyvinylpyrrolidone （PVP） on human urinary bladder carcinoma cells T24 in vitro. Methods Human urinary bladder carcinoma cells T24 were incubated in medium containing PVP in final concentrations of 2.5%, 5.0% and 7.5%, respectively, for 72 h. The MTr test was used to assay the inhibitory effect of PVP on the T24cells while flow cytometry was adopted to examine the influence of PVP on the cell cycle and adhesive property of the carcinoma cells. Results The inhibition rates of the cell growth were （ 15. 11 ± 2.36） % and （49.57 ± 7.07） %, when the cells had been incubated with 2.5% PVP for 24 and 72 h, respectively. The rates increased to（ 35.42 ± 5.55）% and（79.66 ± 19.92）% when the cells had been incubated with 7.5% PVP for 24 and 72 h, respectively. After the T24 cells had been incubated with 5% PVP for 24 and 72 h, the ratios of cells is G0/G1 phase were （74. 17 ± 0.91 ） % and （46.69 ± 3.76） % and those in G2/M phase cells were （ 14.63 ± 0.47） % and （41.88 ± 1.50） %, respectively. 5% PVP could also enhance the effect of murine IgG1 to adhere to the T24 cells. Conclusion PVP was shown to inhibit the growth and proliferation of human urinary bladder carcinoma cells T24 in vitro probably by blocking the cell cycle at the G2/M phase and serving as adhesive carrier of chemotherapeutic agents.
出处
《医药导报》
CAS
2007年第2期124-126,共3页
Herald of Medicine
关键词
聚维酮
膀胱癌
细胞周期
黏附
Polyvinylpyrrolidone
Urinary bladder carcinoma, human
Cell cycle
Adhesion
