摘要
建立了一种简便、快速、准确的测定北五味子中五味子甲素和乙素含量的高效液相色谱分析方法。色谱条件:日本KYAHIQsilC18柱(250mm×4.6mm,5μm),流速1.0mL/min,检测波长250nm,进样量15μL。梯度洗脱程序:乙腈/水溶液在30min内由65%线性升高为85%,保持2min,随后在2min内线性降为65%,平衡5min后等待再次进样。样品制备:以100%甲醇超声波(40kHz)提取北五味子样品80min。用上述方法测定了北五味子根、茎叶和果实中的五味子甲素及乙素的含量。结果显示2种有效成分的含量在果实中最高、茎叶次之、根中最低;五味子甲素在茎叶与果实中含量基本相当,茎叶作为果实的替代入药部位具有可利用基础。
A simple, rapid and sensitive method for the determination of deoxyschizandrin and schisandrin B in Schisandra chinensis （ Tuecz. ） Baill. was established. Bioactive compounds were ultrasonically extracted under the frequency of 40 kHz with 100 % methanol for 80 min, and determined by high performance liquid chromatography. The HPLC separation was preformed on a KYA HIQ sil C18 column （250 mm × 4.6 mm, 5 μm） at flowrate 1 mL/min and detective wavelength 250 nm, the solvent gradient program was as follow： acetonitrile/water increased linearly from 65 % to 85 % in the first 30 min, then isocratic for 2 min, acetonitrile/water decreased linearly from 85 % to 65 % in 2 min; at time 39 min, stopped the program （ ready for next injection）. Deoxyschizandrin and schisandrin B contents in the root, stem and leaves, and fruits of S. chinensis were determined. The results showed that deoxyschizandrin and schisandrin B were highest in the fruits, then in the stem and leaves, and lowest in the root. Deoxysehizandrin content in stem and leaves was equal to fruits, which may suggest that stem and leaves has the value to be explored as the medicinal substitute of fruits of S. chinensis.
出处
《林产化学与工业》
EI
CAS
CSCD
2007年第1期85-88,共4页
Chemistry and Industry of Forest Products
关键词
北五味子
五味子甲素
五味子乙素
超声波提取
Schisandra chinensis
deoxyschizandrin
schisandrin B
ultrasonic extraction
