摘要
本文系统地介绍了金属表面增强荧光现象、产生机理及其应用。粗糙金属和超薄光滑金属表面均可表现出荧光增强效应,但这两种表面增强荧光产生的机理不同,所发出的荧光性质也不同。粗糙金属表面增强荧光的主要原因被认为是加快了处于基质表面附近的荧光物种的辐射衰减过程,而超薄光滑金属表面增强荧光则是激发态荧光物种与金属表面的等离子体耦合共振的结果。与粗糙金属表面增强荧光不同,超薄光滑金属表面增强荧光具有突出的方向性。金属表面增强荧光已经开始在DNA无损检测、荧光共振能量转移免疫分析等领域获得重要应用。
The phenomenon, mechanisms and applications of metallic surface-enhanced fluorescence are reviewed. The phenomenon of enhanced fluorescence emission can be observed on both a non-continuous metallic surface and a continuous metallic surface. But the nature of the enhancement is different. The presence of a non-continuous metallic surface can have dramatic effects on the radiative decay rate of a nearby fluorophore. Above a continuous metallic surface, however, excited fluorophores can couple with surface plasmons, and result in enhanced fluorescence. Compared with the emission near a non-continuous metallic surface, the emission near a continuous metallic surface is highly directional. Furthermore, surface-enhanced fluorescence has found useful in DNA assays, and FRET-based immunoassays.
出处
《化学进展》
SCIE
CAS
CSCD
北大核心
2007年第2期256-266,共11页
Progress in Chemistry
基金
国家自然科学基金项目(No.20373039
20543002)
教育部项目(No.20040718001
306015)资助
关键词
金属增强荧光
辐射衰减工程
辐射等离子体模型
表面等离子体耦合发射
metal enhanced fluorescence
radiative decay engineering
radiating plasmons model
surface plasmon-coupled emission
