摘要
以D-MEM和M199为基础培养基,对菲律宾蛤仔的外套膜进行了细胞培养,并以RNA/DNA比值作为评价细胞增殖的指标。结果表明:外套膜细胞增殖的主要形式是从组织块中迁出,培养前期细胞分泌旺盛,细胞在体外生长可达15 d;外套膜细胞在D-MEM中的生长优于M199;RNA/DNA指标与直观观察的细胞生长状况相吻合,利用这一指标,有助于客观评价体外培养菲律宾蛤仔细胞的增殖特性。
The mantle tissues of clam Ruditapes philippinarum were primary cultured in vitro with Dulbecco＇s Modified Eagle Medium （D-MEM） and Medium 199（ M 199 ）, RNA/DNA ratio was used to evaluate the proliferation of cells. Results showed that the proliferation of mantle cells mostly migrated out from the tissue block, and cells secreted exuberantly in prophase. The culture of mantle cells could maintain 15 days. The effect of D -MEM exceeded M199 in the growth of mantle cells, Moreover, RNA/DNA ratio was consistent with the result observed by microscope. Therefore, RNA/DNA ratio was a good external index to evaluate the proliferation characteristic of clam cells in vitro culture.
出处
《安徽农业科学》
CAS
北大核心
2007年第5期1375-1376,共2页
Journal of Anhui Agricultural Sciences
基金
鲁东大学研究生创新基金项目(Ycx0611)
关键词
菲律宾蛤仔
细胞培养
外套膜
Clam Ruditapes philippinarum
Cellcuhure
Mantle
