摘要
目的探讨食管鳞癌组织中缺氧诱导因子-1α(HIF-1α)和survivin的表达及其临床意义。方法采用免疫组织化学SP法检测50例食管鳞癌组织中HIF-1α和survivin蛋白表达,分析蛋白表达与临床病理特征及预后的关系。结果HIF-1α、survivin蛋白阳性表达率分别为68%和72%,两者存在正相关关系(γ=0.623,P<0.001)。HIF-1α、survivin表达与食管鳞癌临床分期、远处转移密切相关(P<0.05),HIF-1α的表达还与淋巴结转移有关(P<0.05);两者表达均与病理类型、病变长度无显著相关性(P>0.05)。HIF-1α表达阳性和阴性组放疗后完全缓解率及中位生存期分别为8.8%、10个月和81.3%、25个月,两组比较有显著性差异(P≤0.001);1、2、3年生存率分别为38.2%、6.0%、2.9%和81.3%、54.2%、7.9%,Log-Rank检验两组生存曲线有非常显著差异(P=0.001)。结论食管癌组织中存在HIF-1α、survivin蛋白表达,两者表达呈正相关关系,HIF-1α表达与食管鳞癌的放射抗拒性密切相关。
Objective： To explore the expressions of hypoxia inducible factor-1α （HIF-1α）and survivin in esophageal squamous cell cancer （ESCC） and their clinical significance. Methods： The expression of HIF-1α and survivin in 50 patients with ESCC who received radiotherapy was deteced by immunohistoehemical SP method. The relationship between protein expressions and clinicopathological features and prognosis were analyzed. Results： The positive rates of HIF-1α and survivin protein expression were 68% and 72% , respectively. There was a positive relationship between them （ γ = 0. 623, P 〈 0. 001 ）. The expressions of HIF-1α and survivin were closely related with clinical stage and distant metastasis. The expression of HIF-1α correlated with lymph node metastasis. The expressions of HIF-1α and survivin did not correlate with the degree of differentiation and the length of the primary tumor （ P 〉 0. 05 ）. The complete effective rate and mean survival period after radiotherapy were 8.8% and 10 months and 81.3 % and 25 months in HIF-1α-positive and HIF-1α-negative group, respectively （P =0.000）. The 1-, 2-, 3-year survival rates were 38.2%, 6.0% and 2.9% and 81.3%, 54.2%, and 7.9% in HIF-1α-positive and HIF-1α-negative group, respectively. Log-rank test suggested that the difference was significant between HIF-1α-positive and HIF-1α-negative group （P=0. 001 ）. Conclusion： HIF-1α and survivin were overexpressed in ESCC. The expression of HIF-1α protein positively correlates with expression of survivin protein. The expression of HIF-1α has a close relationship with radiotherapy resistance of ESCC.
出处
《肿瘤》
CAS
CSCD
北大核心
2007年第2期139-141,154,共4页
Tumor
基金
河北省科技厅科技攻关课题(编号:052761764)
