摘要
目的:研究褪黑素对病毒唑体外抗呼吸道合胞病毒(RSV)的协同作用。方法:采用MTT比色法,观察2种药物联用时对RSV在人喉癌上皮细胞(Hep-2)所致细胞病变的抑制效应,统计学及金正均法q值判断2种药物的相互作用。结果:病毒唑对RSV具有抑制作用,且呈浓度依赖的趋势;褪黑素几乎没有抗RSV活性,但能增强病毒唑对RSV的抑制作用;2种药物合用对病毒的抑制作用明显增强,褪黑素(25、50mg·L-1)分别与病毒唑(50、100、200mg·L-1)合用时,q值均>1.15,呈现协同作用。结论:病毒唑与褪黑素联合可协同增强对RSV的抑制作用。
OBJECTIVE ：To study the effect of melatonin in enhancing anti - respiratory syscytical virus （RSV） activity of ribavirin in vitro. METHODS： The anti - RSV effect on Hep - 2 cell line of melatonin and ribavirin in combination use was observed by MTT colorimetric assay, and the interaction of melatonin and ribavirin was estimated by q value provided by Jin Zhengjun＇s method. RESULTS： Ribavirin was effective in inhibiting RSV, and the effect was improved with its concentration increasing; melatonin has little activity on RSV resistance,but it can enhance the anti- RSV effect of ribavirin. The inhibition of MT and ribavirin on RSV was significantly enhanced,and the synergistic effect was observed when melatonin （25, 50mg ·L^-1） was used in combination with ribavirin （50,100, 200mg·L^-1）, with q values〉 1.15. CONCLUSIONS： Ribavirin is effective in inhibiting RSV in vitro and the effect is better when used in combination with melatonin.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第7期502-505,共4页
China Pharmacy
