摘要
以银中杨叶片为外植体,建立了高效的再生体系。经正交试验筛选出银中杨组织培养的最适基本培养基为MS培养基,最佳不定芽诱导培养基为MS+BA1.0mg.L-1+NAA0.05 mg.L-1,最佳暗培养时间为4d,最佳不定芽生根培养基为1/2 MS+NAA0.05 mg.L-1+IBA0.01mg.L-1。
The high frequency regeneration system of leaf- explant for Populus alba x P. berolinensis was established through the application of orthogonal designing in this study. The optimal basic medium was MS;the optimal medium for shoot inducement was MS＋ BA1.0mg·L^-1 ＋ NAA0.05 mg·L^-1 , and the optimal time of leaf- explant cultured in dark condition for shoot differentiation was 4 days; the optimal medium for root inducement was 1/2 MS＋ NAA0.05 mg·L^-1 ＋ IBA0.01mg·L^-1 .
出处
《辽宁林业科技》
北大核心
2007年第1期24-27,共4页
Liaoning Forestry Science and Technology
关键词
银中杨
叶片外植体
再生体系
Populus alba ×P. berolinensis
leaf- explant
regeneration system
