摘要
本文采用双抗体夹心ＥＬＩＳＡ法分别测定了２１例反复呼吸道感染儿，３０例正常儿童，１０例新生儿脐血的血清可溶性白细胞介素２受体（ｓＩＬ＝２Ｒ）水平。结果患儿组ｓＩＬ－２Ｒ为７１６．６０±３０．１０Ｕ／ｍｌ；正常儿童组为３８４．４７±８８．０３Ｕ／ｍｌ（ｐ＜０．０１）；新生儿脐血为４４６．２０±５５．６８Ｕ／ｍｌ，与正常儿童比较Ｐ＞０．０５。同时采用间接免疫荧光技术测定了患儿Ｔ细胞亚群水平，结果ＣＤ８细胞数升高，ＣＤ３细胞和ＣＤ４细胞数、ＣＤ４／ＣＤ８比值下降，与正常儿童比较有显著性差别。提示反复呼吸道感染儿有细胞免疫功能降低及免疫调节紊乱。
The serum levels of soluble IL-2 receptor(sIL-2) were determined by double antibody sandwich ELISA in 20 children with recurrent respiratory intection(RRI), 30 normal children and 10 newborn infants. The results showed that sIL-2R concentrations in sera of healthy controls. The levels of sIL-2R in umbilical sera from newborn infants were not significantly different with those of normal controls. In addition, the numbers of T lymphocytes subsets in patients were measured by indirect immunofluorescent technique. The level of CD8 cells were increased while CD3 cells,CD4 cells and the ratio of CD4 to CD8 were decreased. There were statistically significant differences between the groups of patinets with RRI and normal children. Our findings further suggest the reduction of cellular immune functions and disturbances of immune regulation in children with RRI.
出处
《上海免疫学杂志》
CSCD
北大核心
1996年第6期353-354,共2页
Shanghai Journal of Immunology
