摘要
为测定表达犬瘟热病毒(CDV)H基因的重组犬2型腺病毒(CAV-2/CDVLPH)免疫原性,25只45-50日龄犬(抗CDV中和抗体小于1∶2,CAV-2 HI抗体效价小于1∶2)随机分为5组进行免疫犬试验,第1组为CAV-2/CDVLPH免疫组,第2组为pVAXLPH免疫组,第3组为pVAXLPH和CAV-2/CDVLPH联合免疫组,CDV弱毒疫苗组和pVAX1空载体组分别作为阳性和阴性对照组。利用间接ELISA和CDV中和试验检测免疫犬的抗CDV体液免疫水平、CAV-2 HI试验检测免疫犬的抗CAV体液免疫水平、淋巴细胞转化试验检测免疫犬的抗CDV和抗CAV-2细胞免疫水平,分析CAV-2/CDVLPH的免疫性。结果,能够在各试验组免疫犬血清中检测到抗CDV ELISA抗体和抗CDV中和抗体,同时在第1组和第3组免疫犬的血清中检测到抗CAV-2的HI抗体,免疫犬的外周淋巴细胞对特异性和非特异性抗原刺激均有明显增殖,第2组的免疫应答水平低于第3组,第1组又高于第3组,但低于CDV弱毒疫苗免疫组,试验表明CAV-2/CDVLPH可同时诱导免疫犬产生抗CDV和CAV特异性免疫,CAV-2/CDVLPH与pVAXLPH相比能诱导犬产生较高的特异性免疫应答,然而低于CDV弱毒疫苗。
In order to detect the immunity of the recombinant virus expressing canine distemper virus H Gene based on canine adenovirus type-2, twenty five 45-60 day-old dogs （CDV SN〈1 ： 2,CAV-2 HI〈1： 2） were divided into five groups randomly, the first group was inoculated with CAV-2/CDVLPH, the second group with pVAXLPH, the third group with pVAXLPH and CAV-2/CDVLPH, and the group with attenuated CDV vaccine as positive control , the group with empty vector DNA pVAX1 as negative control. Indirect ELISA and neutralization assays were used to detect CDV ELISA antibody and CDV SN antibody of the inoculated dogs, respectively. CAV-2 HI assay was used to detect CAV HI antibody of the inoculated dogs. And lymphocyte transformation test was used to detect the proliferation of T lymphocytes in peripheral blood lymphocytes of the inoculated dogs. The humoral and cellular immune responses against CDV and CAV of the CAV-2/CDVLPH were evaluated. As a result, CDV serum ELISA antibody and CDV SN antibody both were detected in the immunized dogs, and the anti-CAV-2 HI antibody was detected in the dogs of the first group and the second group. And the PBMCs against ConA, CDV or CAV-2 stimulation of the immunized dogs obviously proliferated. The immune reaction against CDV induced by the second group was weaker than that induced by the third group, and that induced by the first group was stronger than that induced by the third group , but weaker than that induced by the CDV modified vaccine. It demonstrated that the dogs inoculated with CAV-2/CDVLPH developed humoral and cellular immune responses against CDV and CAV. The immune reaction against CDV induced by CAV-2/CDVLPH were stronger than that induced by pVAXLPH, but weaker than that induced by the CDV modified vaccine.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2007年第3期258-262,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
"十五"军队医药卫生重点项目(Z2001095)
国家林业局野生动植物保护专项经费资助
