摘要
目的研究P63、P504S和Survivin在前列腺病变组织中的表达情况以及三种标记物联合应用的诊断价值。方法采用联合免疫组化的方法,对94例不同前列腺病变组织进行P63、P504S和Survivin联合检测。结果30例前列腺良性增生(BPH)组织基底细胞的细胞核P63染色皆呈阳性,前列腺上皮细胞的胞浆P504S和Survivin染色皆呈阴性。61例前列腺癌(PCa)组织基底细胞的细胞核P63染色均呈阴性,细胞浆P504S染色均呈阳性,其阳性表达率为95.31%(61/64);44例前列腺上皮细胞的胞浆Survivin染色呈阳性(包括两例局部显示阳性),其阳性表达率为68.75%(44/64)。三种标记物在BPH和PCa组织中的阳性表达率存在显著差异(P<0.05)。前列腺癌中P504S阳性表达强度明显高于Survivin,同时发现Survivin在癌术后转移组织中的表达较P504S有增强的趋势。结论P63阴性表达和P504S阳性表达是诊断前列腺癌的重要指标,而Survivin阳性表达趋势可能在鉴别诊断原发性和继发性前列腺癌方面具有重要意义,因此P63、P504S和Survivin联合检测可提高前列腺癌诊断的准确性。
Objective To investigate the expression of the P63, P504S and Survivin in prostate and their diagnostic value of the combining detection. Methods Combining Immunohistochemistry was used to detect the expression of P63, P504S and Survivin in 94 cases of differem prostate lesions. Results All the P63 colorations of the basal nucleoli were positive in 30 cases of benign prostatic hypertrophy（BPH） tissues and their P504S and Survivin colorations of the epithelia plasma were negative. All the P63 colorations of the basal nucleoli were negative and the P504S colorations of the prostate epithelia plasma were positive and the positive rate was 95.31% （61/64） in 61 cases of prostatic carcinoma （PCa） tissues; but the Survivin colorations of the prostate epithelia plasma were positive in 44 cases （including two partial positive cases）, the positive rate was 68.75% （44/64）. Sensitive difference was discovered between the expressions of the three markers in BPH and PCa tissues （P〈0.05）. The positive rate of the P504S was obviously higher than the Survivin. The expression of Survivin in the metastatic tissues after operation showed a trend of increase in the operated cases whereas the P504S did not. Conclusion The negative expression of the P63 and the positive expression of the P504S are the important markers for the diagnosis of prostate cancer. The positive expression trend of Survivin is very important in the differential diagnosis between primary PCa and secondary PCa. The combining detection of the P63, P504S and Survivin can improve the accuracy in the diagnoses of PCa.
出处
《中国男科学杂志》
CAS
CSCD
2007年第2期21-24,共4页
Chinese Journal of Andrology
基金
湖南省怀化市科学技术局基金资助项目(2005)19号
