摘要
目的探讨革兰阳性球菌的耐药现状,为临床用药提供依据。方法对2001年1月-2005年12月临床各类标本,318株革兰阳性球菌的分离与鉴定严格按《全国临床检验操作规程》进行,体外耐药检测采用琼脂扩散法(K-B法),用美国临床实验室标准化委员会(NCCLS)1999年标准判读药敏结果。结果318株革兰阳性球菌中金黄色葡萄球菌(SAU)居首位占40.3%;其次为凝固酶阴性葡萄球菌(CNS),占21.7%;粪肠球菌占21.1%,居第3位;耐甲氧西林金黄色葡萄球菌(MRSA)检出率为32.8%;耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)检出率为29.0%;药敏结果显示,革兰阳性球菌对常用抗菌药物的耐药率低于深圳、北京、上海、武汉、广州等城市。结论必须重视革兰阳性球菌的耐药检测工作,预防耐药菌株的传播与流行。
OBJECTIVE To investigate the drug resistance of Gram-positive organisms infection in our hospital and offer scientific basis for reasonable usage of antibiotics. METHODS The 318 strain organisms were isolated from the specimens of Aikang Hospital since Jan 2001 to Dec 2005. Gram-positive organisms were isolated from the specimens by the routine methods. The drug susceptivity was tested by K-B method. We got the results by 1999 standard of NCCLS. RESULTS Among the 318 strains, Staphylococcus aureus were 128 strains, 40.3% of the total organisms; coagulase-negative S. aureus were 69 strains, 21. 7% of the total organisms; Enterococcus faecalis were 67 strains, 21. 1% of the total organisms; meticillin-resistant S. aureus（MRSA） were 32. 8%; Meticillin-resistant coagulase-negative S. aureus （MRCNS） were 29. 0%. The results of drug sensitivity test showed the drug resistant rate of Gram-positive strains for commonly used antibacterials was lower than in Shenzhen, Beijing, Shanghai, Wuhan, Guangzhou and other cities. CONCLUSIONS We should attach in our city importance to supervise the drug-resistance of Gram-positive organism, and preclude dissemination and epidemic of drug resistant strains.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2007年第3期328-330,共3页
Chinese Journal of Nosocomiology
关键词
革兰阳性球菌
耐药性
检测
Gram-positive organisms
Drug resistance
Detection
