摘要
目的:探讨黄芪甲苷(ASⅣ)对培养的心肌细胞氧化损伤的保护作用及其机制。方法:体外培养的新生Wistar大鼠心肌细胞分为对照组、0.1%二甲基亚枫(DMSO)溶剂组、H2O2损伤组(0.2 mmol.L-1)、阳性药黄芪注射液组及ASⅣ小、中、大(3、10及30 mg.L-1)剂量组。药物预先处理心肌细胞30 min后,加入H2O2损伤心肌细胞24 h,观察ASⅣ对心肌细胞形态学的影响,MTT法测定心肌细胞存活力以及测量乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、丙二醛(MDA)和一氧化氮(NO)含量的变化。结果:H2O2损伤组心肌细胞部分皱缩,细胞核暗淡,伪足消失;ASⅣ各剂量组心肌细胞损伤程度减轻,心肌细胞核折光性较H2O2损伤组好,伪足略变细;ASⅣ各剂量组细胞存活力均高于H2O2损伤组(P<0.05或P<0.01);ASⅣ10及30 mg.L-1剂量组LDH、MDA含量低于H2O2损伤组(P<0.05或P<0.01),SOD、NO含量高于H2O2损伤组(P<0.05或P<0.01)。结论:ASⅣ可对抗H2O2对心肌细胞的损伤,其机制与提高心肌细胞抗氧化损伤能力、诱导NO生成有关。
Objective To investigate the protective effects of Astragaloside Ⅳ （ASⅣ） on cardiomyocytes against hydrogen peroxide （H2O2） -induced injury. Methods Cultured neonatal rat cardiomyocytes were divided into control group; 0. 1%DMSO solven group; H2O2 group, in which cells were treated with H202 （ 0. 2 mmol · L^-1） for 24 h; ASIV ＋ H2O2 groups, in which cells were pretreated with ASIV （with final concentration of 3, 10, 30 mg · L^-1） for 30 min before H202 treatment; Astragaloside injection group （150 g · L^-1）. Morphological changes of myocardial cells damaged for 24 h by 0.2 mmol · L^-1 H2O2 under the inverted microscope were observed. The cardiomyocyte viability was analysed by MTT assay; lactate dehydrogenase （LDH） activity, superoxide dismutase （SOD） activity, malonaldehyde （MDA） content and nitric oxide （NO） content were detected in culture media. Results Most of cells in H2O2 group were crenulated, the nucleus were dim and the pseudopodia were disappeared. The degrees of lesion in ASN （3, 10, 30 mg · L^-1） groups were lessened obviously, the nucleus were bright and the the pseudopodia were thinned slightly. Cardiomyocyte viabilities in ASN （3, 10, 30 mg · L^-1） groups were higher than that in H202 group （P〈0. 05 or P〈0. 01）. LDH activity and MDA content in ASIV （10, 30 mg .L-1） groups were lower than those in H2O2 group （P〈0. 05 or P〈0. 01）, and SOD activity and NO content in ASN （10, 30 mg · L^-1） groups were higher than those in H2O2 group （P〈0.05 or P〈0.01）. Conclusion AS can protect cardiomyocytes from H2O2 injury by improving cell antioxidant ability and increasing NO content.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第2期211-214,共4页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(30472020)
关键词
黄芪
心肌/细胞学
过氧化氢
一氧化氮
Astragalus membranaceus
myocardium/ cytology
hydrogen peroxide
nitric oxide
