摘要
目的观察通络宁心方对人脐静脉内皮细胞分泌作用的影响。方法体外培养人脐带静脉内皮细胞;分别制备通络宁心方煎剂、复方丹参滴丸含药动物血清,随机分为正常组、模型组、治疗组(大、中、小剂量)、对照组(大、中、小剂量)8组。除正常组外,其余7组予H2O2造模,治疗组、对照组分别予大、中、小剂量含药血清,分别检测一氧化氮(NO)、内皮素-1(ET-1)、P-选择素(Ps)指标。结果模型组NO含量显著低于正常组、治疗及对照大、中、小剂量组;模型组ET-1含量显著高于治疗及对照大、中、小剂量组;模型组Ps含量显著高于正常组,治疗中、小剂量组,对照大、中剂量组,与治疗大剂量、对照小剂量组比较,无显著性差异。结论通络宁心方对血管内皮细胞分泌作用的影响与复方丹参滴丸有同一性,均能对抗H2O2对血管内皮细胞的损伤。
Objectives To observe the protecting effect of Tongluoningxinfang decoction on human umbilical vein endothelial cells. Methods To cultivate endothelial cells and prepare serum with Tongluoningxinfang decoction and Fufangdanshenwan, cells were randomly separated into 8 groups, normal group, model group, treat group （high dose, medium dose, low dose）, control group （high dose, medium dose, low dose）. Except normal group, add 30% H2O2 to the other groups, and make endothelial cells damaged models. Add serum with drugs to treat groups and control groups, and test NO, ET-1 and P-selection （Ps） respectively. Result NO of model group was significantly lower than normal group, all treat groups and all control groups. ET-1 of model group was significantly higher than all treat groups and all control groups. Ps of model group was significantly higher than normal group, treat groups （smedium dose, low dose） and control groups （high dose, medium dose）. It had no significant difference compared with model group, treat group （high dose） and control group （low dose）. Conclusion Tongluoningxirffang decoction has the same influence as Fufangdanshenwan on secretion of vein endothelial cells. Tongluoningxinfang decoction and Fufangdanshenwan all can prevent vein endothelial cells from damage induced by H2O2.
出处
《中国中医药信息杂志》
CAS
CSCD
2007年第4期31-33,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
江苏省中医药管理局重点资助项目[H-008(01-02年度)]
关键词
通络宁心方
人脐静脉内皮细胞
实验研究
Tongluoningxinfang decoction
human umbilical vein endothelial cells
experimental research
