摘要
目的初步探索露蜂房对淋巴细胞与胰岛混合培养系统中淋巴细胞转化的影响。方法露蜂房水提取液系利用其生药通过水提醇沉法制备获得。无菌制备大鼠淋巴细胞及猪胰岛组织悬液,并对二者进行混合培养,在37℃、5%CO2的培养箱中进行,培养过程中分别加入3种浓度(4.0、0.4及0.2g/ml)的露蜂房水提取液,即露蜂房Ⅰ、Ⅱ及Ⅲ组,应用3H-TdR掺入法测定放射性核素每分钟脉冲数(cpm),观察大鼠淋巴细胞的转化情况,并设立空白组和环孢素A(CsA)组作为对照。结果3种浓度的露蜂房水提取液对大鼠淋巴细胞的转化均有明显抑制作用(P<0.001),3组抑制率分别为45.3%、29.6%和9.2%;但与CsA相比,免疫抑制作用较弱(CsA抑制率为80.7%)。结论露蜂房对淋巴细胞胰岛混合培养系统中的淋巴细胞转化有抑制作用,且随浓度增加抑制作用增强,提示露蜂房可以抑制T细胞介导的免疫功能。
Objective To investigate the effect of nidus vespae on lymphocyte blastisation in mixed culture system of lymphocyte and pancreatic islet. Methods Solution of nidus vespae was extracted with ethanol from its herb. Rat lymphocyte and pig pancreatic islet were isolated and then were mixed together and cultured in incubators of 37 ℃ （concentration in volume： 5% CO2）. Three different concentrations of extracted solution of nidus vespae （experimental group Ⅰ ： 4.0 g/ml, group Ⅱ ： 0.4 g/ml, group Ⅲ ： 0.2 g/ml） were added to the mixed culture system of lymphocyte, respectively. Radioactive nuclide counts per minute were measured by ^3 H-thymdine test in order to examine the role of nidus vespae in inhibiting blatisation of lymphocyte, and the results were also compared with control group and CsA group, respectively. Results The counts were all reduced significantly （P〈0. 001） in 3 experimental groups compared with control group （group Ⅰ 45.3%, group Ⅱ 29.6% and group Ⅲ 9.2%）. It also showed that the inhibitory effect became stronger in higher concentration but still weaker than that in CsA group （80.7 % ）. Conclusion Nidus vespae could inhibit the blastisation of lymphocyte in mixed culture system of lymphocyte and pancreatic islet, and the effect increased as the the concentration increased, which may suggest that nidus vespae could suppress the rejection induced by T cells.
出处
《中国普外基础与临床杂志》
CAS
2007年第2期168-170,共3页
Chinese Journal of Bases and Clinics In General Surgery
基金
黑龙江省自然科学基金项目(编号:D01-18)~~
关键词
露蜂房
胰岛
混合培养
淋巴细胞转化
排斥反应
Nidus vespae Pancreatic islet Mixed culture Lymphocyte blastisation Rejection
