摘要
利用从自然界中新分离得到的生淀粉酶产生菌-根霉W-08,并首次采用固态和液态相结合的培养方法,使生淀粉糖化酶活量达到72IU/mL,然后以生玉米粉为底物,利用根酶W-09和酿酒酵母Z-06采用同步糖化与发酵工艺,30℃,48h,发酵醪酒精浓度达到21%(V/V),转化率为理论转化率的94.5%。
A novel raw-starch-digesting glucoamylase producer, Rhizopus sp. W-08, was used in a novel fermentation system of solid-state followed by submerged, and high enzyme activity of 72 IU/mL was obtained. In the following simultaneous saccharification and fermentation process, Saccharomyces cerevisiae Z-06 directly converted raw tom flour to ethanol with the concentration of 21% （V/V） at 30℃ after 48h. The conversion efficiency of raw corn flour to ethanol was 94. 5 % of the theoretical ethanol yield.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第2期261-264,共4页
Microbiology China
关键词
酒精
糖化酶
生玉米粉
根霉
酿酒酵母
Ethanol, Glucoamylase, Rraw tom flour, Rhizopus sp. , Saccharomyces cerevisiae
