摘要
用菌丝生长速率法测定了来自浙江宁波、金华、嘉兴等不同地区保护地蔬菜上采集分离的48个灰霉病菌(Botrytis cinereaPers.)菌株对嘧霉胺(pyrimethanil)的抗性,发现高抗菌株4个(占8.33%),中抗菌株2个(占4.17%),低抗菌株6个(占12.5%),敏感菌株36个(占75%)。抗性菌株在无药培养基上经10代继代培养后,再进行抗性评价,发现其抗嘧霉胺特性能稳定遗传。比较抗性水平不同菌株间的菌丝生长情况,发现菌丝生长量和抗性水平无相关性。研究表明,抗嘧霉胺的灰霉菌亚群体已在浙江省茄科蔬菜中形成,各地应根据抗性水平和用药历史,合理更换或轮换使用与嘧霉胺无交互抗性的药剂,以确保病害的有效控制。
Forty-eight strains of Botrytis cinerea Pers. isolated from Solanaceae vegetables in Ningbo, Jinhua, Jiaxing and etc. were evaluated by the method of mycelial growth inhibition for the resistance to fungicide pyrimethanil. The results demonstrated that the resistant strains to pyrimethanil has been found in Zhejiang Province, China, and the frequency for high, middle, and low resistance were 8.33% ,4.17%, and 12.5%, respectively. Resistance to pyrimethanil for B. cinerea was stable after 10 successive subcultures in fungicide free media. There was no significant correlation between the mycelium gro^h and pyrimethanil-resistance or sensitivity. Strategies to avoid the further development and spread of B. cinerea with resistance to pyrimethanil fungicide were discussed.
出处
《浙江农业学报》
CSCD
2007年第2期123-126,共4页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金资助(30571236)
关键词
茄科蔬菜
灰霉病
嘧霉胺
抗药性
Solanaceae vegetables
Botrytis cinerea
pyrimethanil
fungicide resistance
