摘要
通过对鄂烟1号和其雄性不育母本MSB21、保持系B21、父本B37及鄂烟1号不同烟碱转化率亲本所配的4份新鄂烟1号组合的生物碱及烟碱转化率的研究,发现鄂烟1号烟碱转化率高是由于MSB21及B21烟碱转化率高所致,经2代选择,MSB21的2个株系烟碱转化率由原始群体的31.1%下降到6.4%;B21和B37分别由24.8%和12.8%下降至1.5%以下;鄂烟1号高转化率母本所配的2份组合的烟碱转化率及高转化株所占比例均显著高于对照,而中和非转化株母本所配的组合均显著低于对照。说明烟碱转化在遗传上受显性基因控制,可以通过选择非转化株亲本所配的鄂烟1号新组合,显著降低其烟碱转化率、降烟碱含量和以降烟碱为前体的TSNA之一NNN的含量,从而,显著提高其安全性和香味品质。
The Alkaloid and nicotine conversion of burley hybrid E＇ yan 1 as well as its maternal parent MSB21, maintaining line B21 and paternal parent B37 were detected. Three different nicotine converter lines of MSB21 and non-converter lines of B37 were used to produce 4 new crosses following high-converter × non-converter, middle-converter × non-converter and nonconverter × non-converter for estimating frequency distribution of converters and nicotine conversion rate in the population. Resuh showed that the high proportion of converters in E＇ yan 1 was mainly caused by MSB21 and B21. of systematic selections for non-converters in the parental lines, the nicotine conversion rate in the 2nd B21 and B37 were 6.4%, 1.5% and 1.06%, respectively, whereas their original populations had the Through 2 generations generation of MSB21, nicotine conversion of 31.1%, 24.8% and 12.8%, respectively. The proportion of converters and nicotine conversion rate of 2 crosses made by high-converter × non-converter were significantly higher than that of original E＇ yan 1, 2 crosses made separately by middleconverter × non-converter and non-converter × non-converter were significantly lower than original E＇ yan 1. Hence, nicotine to nornicotine conversion level was genetically controlled by dominant gene. Genetic improvement of burley hybrid E＇ yah 1 to decrease nicotine conversion and harmful component NNN was feasible through making new crosses with the non-converter lines of its parent.
出处
《中国烟草学报》
EI
CAS
CSCD
2007年第2期24-28,29-32,共9页
Acta Tabacaria Sinica
基金
国家烟草专卖局
湖北省烟草专卖局资助项目"烟草烟碱转化及生物碱优化技术研究"(110200401013)一部分。
关键词
鄂烟1号
烟碱转化率
非转化株选择
E＇yan 1
nicotine conversion rate
non-converter selection
