摘要
目的对比性研究3种波段电磁辐射、对大鼠海马脑区的损伤病理及相关因子表达情况,为防治措施提供生物学依据。方法126只大鼠随机分为对照组(C)、高功率S波段微波辐照组(S)、高功率X波段微波辐照组(X)、电磁脉冲辐照组(E),C组给予伪照射、余3组分别给予100 mW.cm-2、100 mW.cm-2及6×104V.m-1,辐照后6 h、1 d、3 d、7 d、14 d、28 d活杀,海马组织经HE染色及甲苯胺兰染色后光镜观察病理学改变;SP免疫组织化学染色法检测GFAP、IL-1β、iN-OS的表达;应用图像分析仪进行定量检测。结果辐照后6 h^7 d各辐照组海马神经元变性和凋亡。辐照后3 d,E、S、X组尼氏体积分光密度(IOD)分别为4.44±0.83、2.70±0.76、2.22±0.69,明显低于C组(13.76±4.88,P<0.01)。辐照后6 h各照射组海马神经元超微结构呈缺血性改变、辐照后3 d出现空泡和次级溶酶体,血管间隙增宽水肿;辐照后3 d,S、X组的IL-1β(IOD)分别为1.29±0.71、1.22±0.43,明显高于C组(0.96±0.29,P<0.01)。iNOS分别为1.44±0.60、1.87±0.52、1.69±0.43,明显高于C组(0.65±0.25,P<0.01)。GFAP分别为0.14±0.07、0.16±0.07、0.16±0.07,明显低于C组(0.19±0.09,P<0.05或p<0.01)。结论本实验条件下,3种波段电磁辐照皆可导致大鼠海马组织损伤,GFAP、IL-1β、iNOS因子参与了电磁辐照所致损伤的病理过程;高功率微波作用较电磁脉冲明显,,而X的作用又较S明显。
Objective： To explore the pathological alterations of hippocampus and the expression of injury-related proteins in Wistar rats irradiated by three kinds of band of electromagnetic radiations, thus to provide the biological basis for prevention and therapeutic measures. Methods A total of 126 rats were randomly divided into control group （C） s-band high power microwave （S） group, x-band high power microwave （X） group, and electromagnetic pulse （E） group. The latter 3 groups were irradiated by 100 mW·cm^-2, 100 mW·cm^-2, and 6×10^4 V·m^-1 respectively. The animals were killed 6 h, 1 d, 3 d, 7 d, 14 d, 28 d after irradiation. Hippocampal sections with HE and TB staining were observed for pathological changes under lightmicroscopy. The expression of glia fibrillary acidic protein （GFAP）, IL-1β, iNOS proteins were detected with SP immunohistochemistry and image analysis. Results Lightmicroscopy showed degeneration and apoptosis in hippocampal neurons of three irradiated groups 6 h-7 d after irradiation The integral optical density （IOD） of Nissel bodies in E, S, X group （4.44±0.83, 2.70±0.76, 2.22±0.69 respectively） were significantly lower than in control group （13.76±4.88, P 〈 0.01） 3 d after irradiation. Electromicroscopy showed ischemic changes 6 h after irradiation, and vacuolization, secondary lysozymes, as well as widening and edema of intervascular space 3d after irradiation in three irradiated groups. The IOD of IL - 1β in S, X group （1.29±0.71, 1.22±0.43 respectively） were significantiy higher than in control group （0.96±0.29, P 〈 0.01 ） 3 d after irradiation. And IOD of iNOS, GFAP in E,S,X group （1.44±0.60, 1.87±0.52, 1.69±0.43; 0.14±0.07, 0.16±0.07, 0.16±0.07 respctively） were significantly higher and lower respectively than in control group （0.651±0.25, 0.193±0.092 respectively, P 〈 0.05, P 〈 0.01）. Conclusion Three kinds of band of electromagnetic irradiation all can damage rat hippocampal neurons to different extent in the order： X 〉 S 〉 E, and GFAP, IL - 1β, and iNOS take part in the pathogenesis of electromagnetic irradiation - induced injury.
出处
《解放军预防医学杂志》
CAS
北大核心
2007年第2期82-86,共5页
Journal of Preventive Medicine of Chinese People's Liberation Army
基金
全军"十五"指令性项目课题(No.01L023)
关键词
电磁辐照
海马
病理损伤效应
尼氏体
electromagnetic radiation
hippocampus
pathological injury effect
Nissel body
