摘要
目的:观察应激对大鼠胃黏膜黏液层pH梯度的影响。方法:制作锑微电极,测定标准溶液的电位(mV)值,绘制pH-mV标准曲线。40只大鼠随机均分为4组。对照组不施加应激,其他3组大鼠分别施加水浸束缚应激1、2和4 h后,以生理盐水冲洗胃腔,以0．01 mol／L盐酸溶液酸化胃腔10 min,取腺胃黏膜以锑微电极测定胃黏液层pH梯度。结果:锑微电极在pH相同但组成不同的标准溶液中测定电位结果不同,但在同一组成的标准缓冲系统中pH-mV呈直线相关。应激1 h组黏液层pH梯度明显高于对照组(P<0．05),黏液层胃腔侧pH明显低于对照组(P<0．05),黏液层上皮侧pH与对照组无显著的统计学差异;应激2 h组黏液层胃腔侧pH较对照组无明显差异,但明显高于应激1 h组(P<0．05),与对照组及应激1 h组相比,黏液层上皮侧pH及pH梯度明显降低(P<0．01);应激4 h组黏液层胃腔侧pH明显高于其他3组(P<0．05),黏液层上皮侧pH较应激2 h组无明显变化,但明显低于应激1 h组及对照组(P<0．01),黏液层pH梯度显著低于其他3组(P< 0．01)。结论:胃黏膜维持黏液层pH梯度的功能在应激早期明显增强,而应激后期明显减弱;应激后期胃黏膜上皮层pH明显降低,提示黏膜上皮层明显酸化。
Objective： To observe the influence of stress on maintaining pH gradients of rat gastric mucus. Methods： Antimony microelectrodes were prepared according to the literature reported. The pH-mV curves of antimony microelectrode in different buffers were p[otted. Forty rats were evenly randomized into 4 groups, including control group （without stress）, 1 h-, 2 h- and 4 h- stress groups. Rats in the latter 3 groups received water immersion restraint stress. The gastric cavities in 4 groups were lavaged with normal saline, followed by lavage with 2 ml hydrochloric acid solution （0.01 mol/L） for 10 min, then the glandular mucosa of stomach was sampled and their mucus pH gradients were measured by antimony microelectrodes. Resuits.The voltages determined by antimony microelectrode in different buffers with the same pH value were different, but they showed a linear correlation with pH-mV in the same buffer. Compared with control group, the 1 h-stress group bad an increased pH gradient （P〈0.05） and a decreased luminal-mucus interface pH value （P〈0.05）; the above 2 groups had a similar mucus-epithelial interface pH value （P〉0.05）. Luminal-mucus interface pH value in 2 h-stress group was similar to that in the control group （P〉0.05）, but was higher than that of 1 h-stress group （P〈0.05）. Compared with 2 h-stress group, the mucus-epithelial interface pH value and the pH gradient in the control and 1 h-stress group were significantly higher （P〈0.01）. The luminal-mucus interface pH value in the 4 h-stress group was significantly higher than those of the other 3 groups （P〈0.05）. The mucus-epithelial interface pH in the 4 h-stress group was similar to that in the 2h-stress group, but was lower than those of the control and 1 h-stress groups （P〈0.01）. The pH gradient in the 4 h-stress group was markedly lower than those in the other 3 groups （P〈0.01）. Conclusion： The results suggest that the gastric mucosa has an obviously improved ability in maintaining mucus pH gradient during the early stage of stress, and a decreased ability during the later stage of stress. The decrease of mucus-epithelial interface pH value during the late stage of stress indicates that the gastric mucosa undergoes obvious acidosis.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2007年第4期377-380,共4页
Academic Journal of Second Military Medical University
基金
军队"十一五"医药卫生科研基金科技攻关项目(06G067)~~
关键词
应激
胃黏膜
黏液
胃酸
stress
gastric mucosa
mucus
gastric acid
