摘要
分别应用病毒分离和区分新城疫强弱毒株的RT-PCR技术,对山东各地疑似鸡新城疫感染的25份病料进行了实验室诊断。结果表明:从18份病料中既扩增到了新城疫强毒基因,也分离到了新城疫病毒;3份病料未分离到病毒,仅扩增到了新城疫强毒基因;4份病料分离到了新城疫病毒,但未检测到相关基因。研究表明,将RT-PCR和病毒分离鉴定两种方法相结合,可更好的满足快速、灵敏、准确的新城疫诊断要求。
The reverse transcriptase-polymerase chain reaction （RT-PCR） was developed to differentiate virulent and avirulent NDV strains. The RT-PCR and virus detection and differentiation method were both used to detect virulent NDV in 25 samples of tissue homogenized liquids. 18 samples were positive both by RT-PCR and virology method. The other 4 samples were positive only by virology method. Another 3 were positive only by RT-PCR. It suggested that more reliable diagnosis result would be achieved by using RT-PCR and the classical virus isolation and identification method together.
出处
《家畜生态学报》
2007年第2期82-85,共4页
Journal of Domestic Animal Ecology
基金
山东省科技攻关项目"猪鸡重大疫病的诊断与防制技术研究"
项目编号:022020103
关键词
新城疫
诊断
RT—PCR
病毒分离
New Castle disease
diagnosis
RT-PCR
virus differentiatior
