摘要
目的:探讨谷氨酰胺(Gln)对肝门阻断后肠道损伤的影响及其意义。方法:将雄性Wistar大鼠,随机分为三组:假手术组(A组)、对照组(B组)和实验组(C组)。采用Pringle法进行肝门阻断,持续35min,在肝门阻断前C组大鼠腹腔注射Gln。分别于肝门阻断前及再灌注后2、4、24h,每组各选取10只大鼠,测定肠道组织谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、丙二醛(MDA)的水平,检测血清TNF-α水平以及门静脉血浆内毒素水平。结果:与A组相比,再灌注后B组肠组织中MDA水平增高(P<0.05),而GSH及SOD水平下降(P<0.05),TNF-α及内毒素水平明显增高(P<0.05)。再灌注后2h及4h,C组肠道GSH水平均明显高于B组(P<0.05)。与B组相比,再灌注后C组肠道组织SOD活性明显增高,而MDA水平明显降低(P<0.05);血清TNF-α及内毒素水平均明显降低(P<0.05)。结论:肝门阻断可以造成肠道屏障的破坏;而Gln对肠道屏障具有保护作用,并将减少内毒素易位、炎性因子的释放。
Objectives： To explore the effect of L-glutamine（GIn） on intestinal injury following total hepatic inflow occlusion. Methods： Male Wistar rats were assigned randomly to three groups （n=40）： Group A, sham-operation; Group B, control group, rats were pretreated with 4 ml 0.9% saline intraperitonally twice per day on 5 consecutive days; Group C, rats were pretreated with Gin dissolved in 4 ml 0.9% saline intraperitoneally twice per day on 5 consecutive days. The rats in group B and C underwent total hepatic inflow occlusion for 35min by the pringle＇ s manoeuvre. Ten rats from each group were randomly sacrificed before occlusion and at 2, 4, 24h after reperfusion respectively. The levels of MDA, SOD, GSH in intestine tissue were measured. The levels of serum TNF- α and portal vein endotoxin were detected. Results： Compared with group A, the levels of GSH and SOD decreased after reperfusion （P〈0.05）, but MDA, and TNF-α and endotoxin increased （P〈0.05） in group B. The levels of SOD in intestine in group C increased after reperfusion compared with group B （P〈0.05）, that of MDA, TNF- α and endotoxin decreased （P〈0. 05）. The levels of GSH in intestine increased at 2 and 4h after reperfusion in group C. Conclusion： Total hepatic inflow occlusion might result in the damage of intestinal barrier. Gin presupplement could alleviate the injury of gut after the pringle＇s manoeuvre in some extent, furthermore reduced the translocation of gut-derived endotoxin and release of proinflammatory factors.
出处
《中国现代普通外科进展》
CAS
2007年第2期131-134,共4页
Chinese Journal of Current Advances in General Surgery
关键词
谷氨酰胺
再灌注损伤
缺血
小肠
谷胱甘肽
超氧化酶
Glutamine, Ischemia-reperfusion injury , Intestine, Glutathione, Superoxide dismutase
