摘要
目的观察大鼠无创性肢体预适应(RIP)后对心肌缺血再灌损伤后心肌形态学、梗死面积和心肌基质金属蛋白酶-2(MMP-2)、MMP-9和组织金属蛋白酶抑制剂-1(TIMP-1)的影响。方法通过连续3d,每天1次3个循环下肢无创性5min缺血、5min再灌注,建立RIP模型。将大鼠随机分成3组,心肌缺血再灌组(I/R组)、心肌缺血预适应组(CIP组)和RIP组。以HE染色法观察心肌形态学改变;以红四氮唑染色法确定心肌梗死范围;以免疫组化法测定心肌MMP-2、MMP-9和TIMP-1的水平。结果与I/R组比较,CIP和RIP均能明显改善心肌损害的形态学改变,降低心肌细胞的肿胀、减少间质出血和炎性细胞的浸润;显著降低心肌梗死面积(P<0.01);降低缺血区心肌MMP-2和MMP-9的水平,增加TIMP-1的水平(P<0.05)。结论RIP不仅能改善缺血再灌注损伤大鼠心肌形态学改变、减少心肌梗死面积,而且能降低心肌细胞基质的损伤,起到保护心肌的作用。
Objective AIM To observe the effect of limb atraumatic ischemic preconditioning （RIP） on myocardial morphologic changes, infarct size and matris metalloproteinases following myocardial ischemia -reperfusion （I/R） injury in rats. Methods RIP was performed in rats by a repeated three - cycle （5 min ischemia - 5 min reperfusion） of hind limb everyday for three days. Three experimental groups were included： I/R, CIP and RIP. The morphologic changes were observed by HE dying, myocardial infarct size was measured with triphenyl tetrazolium choride （TTC） , MMP -2, MMP -9 and TIMP - 1 of myocardium in different areas were decided by immunohistochemistry. Results Compared with I/R, the myocardial swelling, interstitial hemorrhage and inflammatory cell infiltrate were decreased, the myocardial infarct size was reduced （ P 〈0. 01 ） after CIP and RIP. Immunohistochemic results showed that the level of MMP -2 and MMP- 9 was higher, and the TIMP - 1 was lower in I/R in ischemic area than that in non - ischemic area, while CIP and RIP could decrease the level of MMP - 2, MMP - 9 and increase the level of TIMP - 1 （ P 〈 0. 05 ） . Conclusion RIP could protect against myocardial injury through reducing infarct size and decreasing myocardial matris injury.
出处
《中国全科医学》
CAS
CSCD
2007年第9期707-710,共4页
Chinese General Practice
基金
天津市自然科学基金(003608411)
关键词
缺血预适应
心肌再灌注损伤
基质金属蛋白酶
心肌保护
Ischemic preconditioning
Myocardial reperfusion injury
Matris metalloproteinases
Myocardium cardio- protection
