摘要
对紫菜多糖提取液中的杂蛋白质去除方法和条件优化进行了研究。采用Sevag法与三氯乙酸(TCA)法去除紫菜多糖中的游离蛋白质,分别研究了不同溶剂体积比、用量、处理温度和时间等主要因子对蛋白质去除的影响。结果表明,Sevag法最佳脱蛋白条件为氯仿∶正丁醇=3∶1,样品:氯仿-正丁醇=3∶1,振荡时间为30 min;三氯乙酸法(TCA法)最佳脱蛋白条件为反应温度80℃、三氯乙酸用量4%、反应时间30 min。两种脱蛋白方法比较发现:三氯乙酸法去除蛋白质质的效果优于Sevag法。
In this paper, the protein removing methods and their effect factors on polysaccharide extraction from Porphyra yezoensis were studied. Sevag method and TCA method were used to remove protein from Porphyra polysaccharide extraction respectively, and effects of different ratios of solvent volume, dosages, treating temperature and time and other main factors on protein removing were studied respectively. The results showed that the optimum conditions of protein removing with Sevag method were： 3：1 ratio of chloroform：nbutanol,3：1 ratio of sample： chloroform-nbutanol, 30 min stirring time; and the optimum conditions of protein removing with TCA were： 80 ℃ temperature, 30 min treating time, 4% trichloroacetic acid. The comparison between Sevag method and TCA method indicated that protein removing efficieny with TCA method was higher than that with Sevag method .
出处
《上海水产大学学报》
CSCD
北大核心
2007年第2期140-143,共4页
Journal of Shanghai Fisheries University
基金
上海科委重点科技攻关计划项目(054319936)
上海教委优势(重点)学科资助项目(Y1101)
关键词
Sevag法
TCA法
紫菜
多糖
蛋白质去除率
Sevag method
TCA method
Porphyra yezoensis
polysaccharide
protein removing
