摘要
通过接种鸡胚成纤维细胞(CEF)、聚合酶链式反应(PCR)技术及特异性单抗的间接荧光抗体反应(IFA),首次从中国地方品系-黄羽肉鸡中分离到J亚群白血病病毒(ALV-J),并对其gp85基因和3′Ter序列及其致病性作了比较分析。结果显示,GD0510A、GD0510B和GD0512的gp85基因编码的氨基酸序列与国内毒株HN0001同源性最高,分别为94.1%、92.5%和95.8%,GD0510A和GD0512的3′Ter核酸序列与国内毒株同源性比较高。分离到的两株ALV-J(GD0510A和GD0512)感染1日龄肉鸡后出现明显生长抑制(P<0.05),并诱发中枢免疫器官法氏囊和胸腺萎缩。两株病毒单独感染均能降低鸡体对新城疫病毒和禽流感病毒(AIV-H5)疫苗的抗体滴度,GD0512感染鸡后能明显抑制感染鸡对新城疫病毒疫苗的免疫反应(P<0.05),而GD0510A感染鸡后在4w时也能明显抑制感染鸡对禽流感病毒(AIV-H5)疫苗的免疫反应(P<0.05)。研究证实在我国地方品系黄羽肉鸡存在ALV-J的感染,分子生物学特性研究表明该毒株可能是来自白羽肉鸡且能造成感染鸡的免疫抑制。
Avian leukosis viruses of subgroup J（ALV-J）were firstly isolated from yellow chickens by inocu- lating the samples into chick embryo fibroblast（CEF）, the PCR amplification of the infected CEF genomic DNA and the indirect fluorescence assay（IFA） with ALV-J specific monoclonal antibody JE-9. The amino acid sequences of gp85 genes of the isolates of GD0510A.GD0510B and GD0512 were highly homologous with that of the chinese strain HN0001, the homogeneity was 94. 1%、92.5% and 95.8% respectively. The sequences of 3＇Ter of them were highly homologous to other Chinese strains. Immunosuppression was detected when 1-day-old commercial broilers were infected with ALV-J strains GD0510A and GD0512, the body weight was significantly reduced（P〈0.05）. Antibody reaction to Newcastle disease virus vaccine was inhibited significantly （P〈0.05） after chickens were infected with GD0512, antibody reaction to AIV- H5 also was inhibited significantly（P〈0.05） on fourth week after chickens were infected with GD0510A. It was the first time to report ALV-J isolation from Chinese local breed of yellow chickens, and it was speculated that these virus strains might come from white chickens according to sequence comparisons.
出处
《病毒学报》
CAS
CSCD
北大核心
2007年第3期207-211,共5页
Chinese Journal of Virology
基金
国家自然科学基金重点项目资助(30330450)
