摘要
目的:检测乳腺癌患者骨髓中的human mamglobin(hMAM)mRNA和CK19mRNA表达,探讨乳腺癌患者早期骨髓微转移与临床病理学因素的关系。方法:采用逆转录-聚合酶链反应(RT-PCR一步法)法检测骨髓组织中hMAM mRNA和CK19mRNA的表达。结果:102例乳腺癌患者骨髓hMAM mRNA、CK19mRNA表达阳性者分别为37.3%和56.9%,均高于良性乳腺疾病患者骨髓组织中的表达(P=0.043,P=0.002)。两者的阳性表达率与肿瘤大小、临床分期和组织学分级呈显著相关,P值分别为0.000、0.001、0.020、0.003、0.031和0.001;与淋巴结转移状况、年龄和月经状况无关,P值分别为0.101、0.255、0.111、0.110、0.492和0.187。结论:乳腺癌微转移与肿瘤大小、临床分期和组织学分级有关,hMAM mRNA和CK19mRNA均可作为检测乳腺癌骨髓微转移标志,且两者间存在一致性。
OBJECTIVE： To detect the expressions of hMAM mRNA and CK19 mRNA in bone marrow（BM） of patients with breast cancer and understand the rehionship between micrometastases in BM and clinicopathological parameters of breast cancer. METHODS： RT PCR（one-step） was used to de tect the expressions of hMAM mRNA and CK19 mRNA in BM of 102 samples of breast cancer. RESULTS： The positive expressions of hMAM（37.3%）and CK19（56.9%）in BM of breast cancer were higher than those in the BM of benign lesions of breast patients（P=0.043, P=0.002, respectively）. A signifi cant correlation was found that the positive expressions of hMAM and CKI9 were related to the size of tumor, clinical stage and bistological differentiation （P= 0. 000,0, 001. 0. 020. 0. 003,0. 031 and 0. 001 ,respectively） ;but not related to lymph node metastasis, age or menopausal status. CONCLUSIONS： The micrometastases in BM are correlated with some pathology parameters, hMAMm and CK19 are in concordance with the. dection of breast cancer micrometastasis in BM.
出处
《中华肿瘤防治杂志》
CAS
2007年第9期676-679,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
河北省科技厅基金(042761100)
关键词
乳腺肿瘤/病理学
骨髓/代谢
淋巴转移
逆转录聚合酶链反应
breast neoplasms/pathology
bone marrow/metabolism
lymphatic metastasis
reverse transcriptasepolymerase chain reaction
