摘要
以98个家系组成的Nipponbare/Kasalath//Nipponbare回交重组自交系及其亲本为遗传材料,利用不同方式GA3处理幼苗以及复合区间作图法,研究了苗高和叶鞘长度对外源GA3处理的敏感性及控制GA3反应指数的数量性状位点（QTL）.结果表明,GA3浸泡处理的幼苗第一叶鞘长度较对照显著增加,苗高和第二叶鞘长度增加效应不显著;GA3喷洒处理对第一叶鞘长度增加不显著,苗高和第二叶鞘长度显著增加.GA3浸泡处理条件下,检测到1个位于第1染色体的控制第一叶鞘长度反应指数的QTL（qIFL-1）,解释性状变异的14%,对GA3敏感的等位基因来自Kasalath.GA3喷洒处理条件下,检测到2个分别位于第3、12染色体的控制第二叶鞘长度反应指数的QTL（qJSL-3和qISL-12）,分别解释5%和20.2%的性状变异,对GA3敏感的等位基因分别来自于Kasalath和Nipponbare.qIFL-1与控制第一叶鞘长度本身的数量性状位点qFSL-1a位于同一染色体区段（C742～R2414）;qISL-12位于控制第二叶鞘长度本身的qSSL-12a与qSSL-12b之间（C732～G193）.
A mapping population with 98 backcross inbred lines （BC1F12）, derived from a backcross of Nipponbare （ssp. japonica）/Kasalath （ssp. indica）//Nipponbare by the single seed descent methods,was employed to study different disposal of GAs in inducing seedling growing and leaf sheath elongating and to map quantitative trait loci （QTL） of GAs responsive index by composite interval mapping method in this research. The result showed that the first leaf sheath length increased significantly than control, but seedling height and the second leaf sheath increased slightly in GAs soaking experiment. In GAs spraying experiment,seedling height and the second leaf sheath length increased significantly, but the first leaf sheath length was similar with that of control. For responsive index of the first leaf sheath length, 1 QTL were detected on chromosome 1,which explained 14.0% of the phenotypic variance. For responsive index of the second leaf sheath length,2 QTLs were detected on chromosome 3 and 12, respectively. Which totally explained 25.2% of the phenotypic variance, qIFL-1 and qFSL-1a located the same marker interval （C742- R2414） ,qISL-12 located between qSSL-12a and qSSL-12b（C732-G193）.
出处
《西北植物学报》
CAS
CSCD
北大核心
2007年第4期684-692,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
长江学者和创新团队发展计划(IRT0432)
关键词
水稻
GA3处理效应
苗高
叶鞘长度
反应指数
数量性状基因座定位
rice
GAs treated effect
seedling height
leaf sheath length
responsive index
quantitative trait locus mapping
