摘要
以鄂西地方玉米巫溪（Ｗ）、美国硬粒坚杆玉米（Ｂ）及墨西哥耐热玉米墨黄九（Ｍ）３个开放授粉群体为主体，加另外８份材料经手配重组和３次隔离区混粉重组合成的用于轮回选择的基础群体ＷＢＭＣｏ等４个开放群体为试材，比较了单株粒重、穗重、株高等１５个农艺性状的平均值及标准差。以穗粒重为因变量进行回归分析，以及用ＲＡＰＤ和ＲＦＬＰ分析技术，在ＤＮＡ水平上进行遗传多态性的初步研究。结果表明，同一性状的均值，在４个群体间存在差异。其中穗长、穗粗、行粒数、穗重、穗粒重、百粒重、轴粗、轴重、出籽率的均值，以ＷＢＭＣｏ最大。同一性状的标准差，在４个群体中以ＷＭＢＣｏ在穗长、穗行数、穗重、穗粒重、百粒重、轴粗、轴重、株高性状变异丰富。４个群体的回归方程中，影响单株籽粒产量的自变量因子效应大小不同。ＲＡＰＤ及ＲＦＬＰ分析较好地揭示了供试材料在ＤＮＡ水平上的遗传变异。组建的ＷＢＭＣｏ群体符合轮回选择基础群体的要求。
The means and standard deviation of grain weight, ear weight, plant height etc., a total of 15 agronomic traits, were compared. Regression analysises were carried out between grain weight and other 14 agronomic traits. RAPD and RFLP molecular techniques were also used to assay genetic diversity at DNA level using 4 openponinated maize populations, which were Wuxi (W) from southern west China, Bsss (B) from American, Suwan (S) from MeXico and the synthetic population (WBMCo) of these 3 parent populations with 8 other materials. The results showed that the means of the same agronomic trait were different among the 4 populations, and WBMCo came first at ear length, ear width, row grain number, ear weight, grain weight, hundred grain weight, core width, core weight and grain percent. Standard deviations of the same agronomic trait were also different. WBMCo presented more diversities at ear length, row number, ear weight, ear grain weight, hundred grain weight, core width and plant height. The regression formular indicated that contributions of independent variable to grain weight were not the same among the 4 populations. Both RAPD and RFLP assay revealed a genetic variance at DNA level. The synthetic population WBMCo is qualified to be a basic population for recurrent selection.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1997年第1期12-17,共6页
Journal of Huazhong Agricultural University
基金
国际IFS及国家自然科学基金
关键词
玉米
群体
遗传变异
maize, population, genetic variance
