摘要
eDNA amplified fragment length polymorphism （cDNA-AFLP） analysis was used to investigate the differentially expressed genes between sterile and fertile plants of ms5ms6 double-recessive genie male sterility （GMS） two-type line cotton （Gossypium hirsutum L.） at different stages, i.e., sporogenous cell stage, pollen mother cell （PMC） stage, and pollen grain stage. Seventeen differentially expressed fragments were identified. Functional analysis indicated that their corresponding genes may participate in the processes of signal transduction, transcription, energy metabolism, and plant cell wall development. Northern blot demonstrated the credibility of the result of cDNA-AFLE A sterility restorer factor-like gene, which only expressed in fertile anther and was notably homologous to T cytoplasm male sterility restorer factor 2 of maize （Zea mays L.）, was identified in this research.
应用cDNA-AFLP对棉花ms5ms6双隐性核雄性不育两用系的不育株和可育株花粉发育的3个时期—造孢细胞时期、花粉母细胞时期和花粉粒时期进行对比分析,共得到17个差异表达片段,它们分别属于11种表达模式,其中14个片段可以在NCBI数据库中找到同源序列,功能分析表明这些片段所编码的基因可能参与了信号转导、转录、能量代谢、细胞壁发育等相关过程。Northern杂交结果证明检测片段的表达模式与cDNA-AFLP结果吻合。同时还在可育花药中发现了与玉米T型细胞质雄性不育恢复因子RF2基因高度同源的育性恢复因子类基因。
基金
This work was supported by Chinese National Program for High Technology Research and Development(No.2001AA7005).
